Pseudomonas aeruginosa PAO1, PA5089 (tle5b)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5089
Name
tle5b
Synonym: pldB
Replicon chromosome
Genomic location 5727239 - 5729476 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253776.1
GI 15600282
Affymetrix PA5089_at
Entrez 882245
GenBank AAG08474.1
INSDC AAG08474.1
NCBI Locus Tag PA5089
protein_id(GenBank) gb|AAG08474.1|AE004921_11|gnl|PseudoCAP|PA5089
TIGR NTL03PA05090
UniParc UPI00000C5EF6
UniProtKB Acc Q9HU94
UniProtKB ID Q9HU94_PSEAE
UniRef100 UniRef100_Q9HU94
UniRef50 UniRef50_Q9HU94
UniRef90 UniRef90_Q9HU94

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion phospholipase D effector Tle5b
Product Name Confidence: Class 1
Synonyms PldB
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.58
Kyte-Doolittle Hydrophobicity Value -0.567
Molecular Weight (kDa) 83.4
Isoelectric Point (pI) 7.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Host Associated Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 66 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004687 (323 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5089
Search term: tle5b

Human Homologs

References

A Pseudomonas aeruginosa type VI secretion phospholipase D effector targets both prokaryotic and eukaryotic cells.
Jiang F, Waterfield NR, Yang J, Yang G, Jin Q
Cell Host Microbe 2014 May 14;15(5):600-10
PubMed ID: 24832454
The T6SSs of Pseudomonas aeruginosa Strain PAO1 and Their Effectors: Beyond Bacterial-Cell Targeting.
Sana TG, Berni B, Bleves S
Front Cell Infect Microbiol 2016;6:61
PubMed ID: 27376031