Pseudomonas aeruginosa PAO1, PA5134 (ctpA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5134
Name	ctpA Synonym: ctpA
Replicon	chromosome
Genomic location	5782278 - 5783588 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	Structural similarity to 4C2E: carboxyprotease from Tetradesmus obliquus (root-mean-square deviation: 1.6 angstroms)

Cross-References

RefSeq	NP_253821.1
GI	15600327
Affymetrix	PA5134_at
Entrez	878610
GenBank	AAG08519.1
INSDC	AAG08519.1
NCBI Locus Tag	PA5134
protein_id(GenBank)	gb|AAG08519.1|AE004926_9|gnl|PseudoCAP|PA5134
TIGR	NTL03PA05135
UniParc	UPI00000C5F1C
UniProtKB Acc	Q9HU50
UniProtKB ID	Q9HU50_PSEAE
UniRef100	UniRef100_W0Z2V8
UniRef50	UniRef50_C5BJ27
UniRef90	UniRef90_A0A0A8RJL6

Product

Feature Type	CDS
Coding Frame	1
Product Name	carboxyl-terminal processing protease, CtpA Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected using LC-MS/MS (46.02 kDa, PMID:21751344)
Charge (pH 7)	-4.29
Kyte-Doolittle Hydrophobicity Value	-0.193
Molecular Weight (kDa)	46.0
Isoelectric Point (pI)	5.36

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543 Periplasmic Class 1 19333994 Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7RPQ	HYDROLASE	08/04/21	Crystal Structure of carboxyl-terminal processing protease A, CtpA, of Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	3.3	X-RAY DIFFRACTION	100.0
7RQH	HYDROLASE	08/06/21	Crystal Structure of carboxyl-terminal processing protease A mutant S302A, CtpA_S302A, of Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	3.2	X-RAY DIFFRACTION	99.7

Virulence Evidence

Source 1
Database	PseudoCAP
Category
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism	Cricetulus griseus
Notes
Infection model	Cytotoxicity assay using chinese hamster ovary (CHO-K1) cells.
Reference	24082078
Source 2
Database	PseudoCAP
Category
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism	Mus musculus
Notes
Infection model	Mouse model of acute pneumonia
Reference	24082078

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 507 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004726 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5134 Search term: ctpA Search term: carboxyl-terminal processing protease, CtpA

Human Homologs

References

A carboxy-terminal processing protease gene is located immediately upstream of the invasion-associated locus from Bartonella bacilliformis. Mitchell SJ, Minnick MF Microbiology (Reading) 1997 Apr;143 ( Pt 4):1221-1233 PubMed ID: 9141685

Transport of CtpA protein from the cyanobacterium Synechocystis 6803 across the thylakoid membrane in chloroplasts. Karnauchov I, Herrmann RG, Pakrasi HB, Klösgen RB Eur J Biochem 1997 Oct 15;249(2):497-504 PubMed ID: 9370359

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

The Pseudomonas aeruginosa periplasmic protease CtpA can affect systems that impact its ability to mount both acute and chronic infections. Seo J, Darwin AJ Infect Immun 2013 Dec;81(12):4561-70 PubMed ID: 24082078

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799