Pseudomonas aeruginosa PAO1, PA5153

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5153
Name
Replicon	chromosome
Genomic location	5801315 - 5802067 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	Structural similarity to 4ZV1 (ancestral arginine-binding protein, root-mean-square deviation: 1.0 angstroms). Sequence similarity to 3TQL (97%/40,97%) (ARG). Final predicted ligand: arginine (PMID: 31631799)

Cross-References

RefSeq	NP_253840.1
GI	15600346
Affymetrix	PA5153_at
DNASU	PaCD00008217
Entrez	879969
GenBank	AAG08538.1
INSDC	AAG08538.1
NCBI Locus Tag	PA5153
protein_id(GenBank)	gb|AAG08538.1|AE004928_4|gnl|PseudoCAP|PA5153
TIGR	NTL03PA05154
UniParc	UPI00000C5F2B
UniProtKB Acc	Q9HU31
UniProtKB ID	Q9HU31_PSEAE
UniRef100	UniRef100_W0Z139
UniRef50	UniRef50_A0A068D026
UniRef90	UniRef90_M4XMN6

Product

Feature Type	CDS
Coding Frame	1
Product Name	amino acid (lysine/arginine/ornithine/histidine/octopine) ABC transporter periplasmic binding protein Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-4.06
Kyte-Doolittle Hydrophobicity Value	-0.232
Molecular Weight (kDa)	27.6
Isoelectric Point (pI)	4.90

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 453 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004746 (530 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5153 Search term: amino acid (lysine/arginine/ornithine/histidine/octopine) ABC transporter periplasmic binding protein

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Physical map location of the new artPIQMJ genes of Escherichia coli, encoding a periplasmic arginine transport system. Wissenbach U, Keck B, Unden G J Bacteriol 1993 Jun;175(11):3687-8 PubMed ID: 8501075

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799