Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5164
|
Name |
rmlC
Synonym: rfbC |
Replicon | chromosome |
Genomic location | 5813122 - 5813667 (+ strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
RefSeq | NP_253851.1 |
GI | 15600357 |
Affymetrix | PA5164_rmlC_at |
DNASU | PaCD00008220 |
Entrez | 879991 |
GenBank | AAG08549.1 |
INSDC | AAG08549.1 |
NCBI Locus Tag | PA5164 |
protein_id(GenBank) | gb|AAG08549.1|AE004929_4|gnl|PseudoCAP|PA5164 |
TIGR | NTL03PA05165 |
UniParc | UPI00000DCE21 |
UniProtKB Acc | Q9HU21 |
UniProtKB ID | RMLC_PSEAE |
UniRef100 | UniRef100_Q9HU21 |
UniRef50 | UniRef50_Q9HU21 |
UniRef90 | UniRef90_Q9HU21 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
dTDP-4-dehydrorhamnose 3,5-epimerase
|
Synonyms |
dTDP-4-keto-6-deoxyglucose 3,5-epimerase dTDP-L-rhamnose synthetase |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -2.89 |
Kyte-Doolittle Hydrophobicity Value | -0.383 |
Molecular Weight (kDa) | 20.8 |
Isoelectric Point (pI) | 6.02 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1RTV | ISOMERASE | 12/10/03 | RmlC (dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase) crystal structure from Pseudomonas aeruginosa, apo structure | Pseudomonas aeruginosa | 2.5 | X-RAY DIFFRACTION | 100.0 |
2IXI | ISOMERASE | 07/08/06 | RmlC P aeruginosa with dTDP-xylose | PSEUDOMONAS AERUGINOSA | 1.8 | X-RAY DIFFRACTION | 100.0 |
6DIN | ISOMERASE | 05/23/18 | High resolutionstructure of apo dTDP-4-dehydrorhamnose 3,5-epimerase | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
2IXH | ISOMERASE | 07/08/06 | RmlC P aeruginosa with dTDP-rhamnose | PSEUDOMONAS AERUGINOSA | 2 | X-RAY DIFFRACTION | 100.0 |
2IXK | ISOMERASE | 07/08/06 | RmlC P aeruginosa with dTDP-4-keto rhamnnose (the product of the reaction) | PSEUDOMONAS AERUGINOSA | 1.7 | X-RAY DIFFRACTION | 100.0 |
2IXJ | ISOMERASE | 07/08/06 | RmlC P aeruginosa native | PSEUDOMONAS AERUGINOSA | 2.54 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
D-tartaric acid | DB01694 | DrugBank | 5.1.4 | Q9HU21 | dTDP-4-dehydrorhamnose 3,5-epimerase | 100.0 | 181 | 3.2e-105 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 473 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004755 (533 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5164
Search term: rmlC
Search term: dTDP-4-dehydrorhamnose 3,5-epimerase
|
Involvement of the rml locus in core oligosaccharide and O polysaccharide assembly in Pseudomonas aeruginosa.
Rahim R, Burrows LL, Monteiro MA, Perry MB, Lam JS
Microbiology (Reading) 2000 Nov;146 ( Pt 11):2803-2814
PubMed ID: 11065359
|
Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence.
Rajakumar K, Jost BH, Sasakawa C, Okada N, Yoshikawa M, Adler B
J Bacteriol 1994 Apr;176(8):2362-73
PubMed ID: 8157605
|
Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a.
Yao Z, Valvano MA
J Bacteriol 1994 Jul;176(13):4133-43
PubMed ID: 7517390
|
RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation.
Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH
J Mol Biol 2007 Jan 5;365(1):146-59
PubMed ID: 17046787
|