Pseudomonas aeruginosa PAO1, PA5167 (dctP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5167
Name
dctP
Replicon chromosome
Genomic location 5817191 - 5818186 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentStructural similarity to 4MX6 (TRAP periplasmic solute binding protein from Shewanella oneidensis, root-mean-square deviation: 0.5 angstroms). Sequence similarity to 4O94 (98%/60,07%) (SIN). Final predicted ligand: succinic acid (PMID: 31631799)

Cross-References

RefSeq NP_253854.1
GI 15600360
Affymetrix PA5167_at
DNASU PaCD00008221
Entrez 879964
GenBank AAG08552.1
INSDC AAG08552.1
NCBI Locus Tag PA5167
protein_id(GenBank) gb|AAG08552.1|AE004929_7|gnl|PseudoCAP|PA5167
TIGR NTL03PA05168
UniParc UPI00000C5F34
UniProtKB Acc Q9HU18
UniProtKB ID Q9HU18_PSEAE
UniRef100 UniRef100_W0Z150
UniRef50 UniRef50_A3QCW5
UniRef90 UniRef90_V6AP12

Product

Feature Type CDS
Coding Frame 1
Product Name
DctP
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 3.42
Kyte-Doolittle Hydrophobicity Value -0.361
Molecular Weight (kDa) 37.1
Isoelectric Point (pI) 8.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 257 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000851 (1023 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5167
Search term: dctP
Search term: DctP

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Purification, characterization and nucleotide sequence of the periplasmic C4-dicarboxylate-binding protein (DctP) from Rhodobacter capsulatus.
Shaw JG, Hamblin MJ, Kelly DJ
Mol Microbiol 1991 Dec;5(12):3055-62
PubMed ID: 1809844
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Identification of C(4)-dicarboxylate transport systems in Pseudomonas aeruginosa PAO1.
Valentini M, Storelli N, Lapouge K
J Bacteriol 2011 Sep;193(17):4307-16
PubMed ID: 21725012
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799