Pseudomonas aeruginosa PAO1, PA5302 (dadX)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5302
Name	dadX Synonym: alr2
Replicon	chromosome
Genomic location	5970686 - 5971759 (- strand)
Transposon Mutants	3 transposon mutants in PAO1
Transposon Mutants in orthologs	6 transposon mutants in orthologs

Cross-References

RefSeq	NP_253989.1
GI	15600495
Affymetrix	PA5302_dadX_at
Entrez	878055
GenBank	AAG08687.1
INSDC	AAG08687.1
NCBI Locus Tag	PA5302
protein_id(GenBank)	gb|AAG08687.1|AE004943_3|gnl|PseudoCAP|PA5302
TIGR	NTL03PA05303
UniParc	UPI00001258D4
UniProtKB Acc	Q9HTQ2
UniProtKB ID	ALR2_PSEAE
UniRef100	UniRef100_Q9HTQ2
UniRef50	UniRef50_Q9HTQ2
UniRef90	UniRef90_Q9HTQ2

Product

Feature Type	CDS
Coding Frame	1
Product Name	catabolic alanine racemase Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-0.77
Kyte-Doolittle Hydrophobicity Value	0.005
Molecular Weight (kDa)	38.9
Isoelectric Point (pI)	6.85

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
1RCQ	ISOMERASE	11/04/03	The 1.45 A crystal structure of alanine racemase from a pathogenic bacterium, Pseudomonas aeruginosa, contains both internal and external aldimine forms	Pseudomonas aeruginosa	1.45	X-RAY DIFFRACTION	99.2

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
Lysine Nz-Carboxylic Acid	DB03801	DrugBank	5.1.4	Q9HTQ2	Alanine racemase, catabolic	100.0	357	1.4e-205
D-Lysine	DB03252	DrugBank	5.1.4	Q9HTQ2	Alanine racemase, catabolic	100.0	357	1.4e-205

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 560 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004545 (850 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5302 Search term: dadX Search term: catabolic alanine racemase

Human Homologs

References

Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of D-amino acid dehydrogenase and the catabolic alanine racemase. Lobocka M, Hennig J, Wild J, Kłopotowski T J. Bacteriol. 1994 Mar;176(5):1500-10 PubMed ID: 7906689

Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1. Strych U, Huang HC, Krause KL, Benedik MJ Curr Microbiol 2000 Oct;41(4):290-4 PubMed ID: 10977898