Pseudomonas aeruginosa PAO1, PA5308 (lrp)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5308
Name
lrp
Synonym: dadR, dadAX regulator
Replicon chromosome
Genomic location 5977123 - 5977611 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253995.1
GI 15600501
Affymetrix PA5308_lrp_at
DNASU PaCD00008292
Entrez 878436
GenBank AAG08693.1
INSDC AAG08693.1
NCBI Locus Tag PA5308
protein_id(GenBank) gb|AAG08693.1|AE004943_9|gnl|PseudoCAP|PA5308
TIGR NTL03PA05309
UniParc UPI00000C5F9B
UniProtKB Acc Q9HTP6
UniProtKB ID Q9HTP6_PSEAE
UniRef100 UniRef100_W0YZ70
UniRef50 UniRef50_P45265
UniRef90 UniRef90_Q4K3T6

Product

Feature Type CDS
Coding Frame 1
Product Name
leucine-responsive regulatory protein
Product Name Confidence: Class 2
Synonyms transcriptional activator of dadAX DadR
Lrp protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.62
Kyte-Doolittle Hydrophobicity Value -0.354
Molecular Weight (kDa) 18.9
Isoelectric Point (pI) 8.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 456 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004880 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5308
Search term: lrp

Human Homologs

References

Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine.
Willins DA, Ryan CW, Platko JV, Calvo JM
J Biol Chem 1991 Jun 15;266(17):10768-74
PubMed ID: 2040596
Transcriptome analysis of agmatine and putrescine catabolism in Pseudomonas aeruginosa PAO1.
Chou HT, Kwon DH, Hegazy M, Lu CD
J Bacteriol 2008 Mar;190(6):1966-75
PubMed ID: 18192388