Pseudomonas aeruginosa PAO1, PA5320 (coaC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5320
Name
coaC
Synonym: coaB
Synonym: dfp
Synonym: coaBC
Replicon chromosome
Genomic location 5989460 - 5990668 (+ strand)

Cross-References

RefSeq NP_254007.1
GI 15600513
Affymetrix PA5320_dfp_at
DNASU PaCD00008297
Entrez 877887
GenBank AAG08705.1
INSDC AAG08705.1
NCBI Locus Tag PA5320
protein_id(GenBank) gb|AAG08705.1|AE004944_9|gnl|PseudoCAP|PA5320
TIGR NTL03PA05321
UniParc UPI00000C5FA4
UniProtKB Acc Q9HTN4
UniProtKB ID Q9HTN4_PSEAE
UniRef100 UniRef100_Q9HTN4
UniRef50 UniRef50_P0ABQ1
UniRef90 UniRef90_A6VEC7

Product

Feature Type CDS
Coding Frame 1
Product Name
Phosphopantothenoylcysteine synthase/(R)-4'-phospho-N-pantothenoylcysteine decarboxylase
Product Name Confidence: Class 2
Synonyms CoaBC protein
Dfp protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.94
Kyte-Doolittle Hydrophobicity Value 0.022
Molecular Weight (kDa) 43.1
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 632 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004891 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5320
Search term: coaC

Human Homologs

Ensembl 110, assembly GRCh38.p14
phosphopantothenoylcysteine decarboxylase [Source:HGNC Symbol;Acc:HGNC:28107]
E-value: 3.6e-16
Percent Identity: 31.6

References

Molecular characterization of lantibiotic-synthesizing enzyme EpiD reveals a function for bacterial Dfp proteins in coenzyme A biosynthesis.
Kupke T, Uebele M, Schmid D, Jung G, Blaesse M, Steinbacher S
J Biol Chem 2000 Oct 13;275(41):31838-46
PubMed ID: 10922366
Phosphopantothenoylcysteine synthetase from Escherichia coli. Identification and characterization of the last unidentified coenzyme A biosynthetic enzyme in bacteria.
Strauss E, Kinsland C, Ge Y, McLafferty FW, Begley TP
J Biol Chem 2001 Apr 27;276(17):13513-6
PubMed ID: 11278255
dfp Gene of Escherichia coli K-12, a locus affecting DNA synthesis, codes for a flavoprotein.
Spitzer ED, Weiss B
J Bacteriol 1985 Dec;164(3):994-1003
PubMed ID: 2999089
beta-Alanine auxotrophy associated with dfp, a locus affecting DNA synthesis in Escherichia coli.
Spitzer ED, Jimenez-Billini HE, Weiss B
J Bacteriol 1988 Feb;170(2):872-6
PubMed ID: 3123465