Pseudomonas aeruginosa PAO1, PA5325 (sphA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5325
Name
sphA
Replicon chromosome
Genomic location 5996036 - 5996986 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254012.1
GI 15600518
Affymetrix PA5325_at
DNASU PaCD00008300
Entrez 880923
GenBank AAG08710.1
INSDC AAG08710.1
NCBI Locus Tag PA5325
protein_id(GenBank) gb|AAG08710.1|AE004945_4|gnl|PseudoCAP|PA5325
TIGR NTL03PA05326
UniParc UPI00000C5FA6
UniProtKB Acc Q9HTN0
UniProtKB ID Q9HTN0_PSEAE
UniRef100 UniRef100_W0Z1I2
UniRef50 UniRef50_Q1I8G0
UniRef90 UniRef90_A6VED2

Product

Feature Type CDS
Coding Frame 1
Product Name
SphA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -7.41
Kyte-Doolittle Hydrophobicity Value -0.263
Molecular Weight (kDa) 35.5
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 2
sequence alignment evidence used in manual assertion
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 60 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003078 (1061 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5325
Search term: sphA
Search term: SphA

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 20702
Genomic Position 5996313
Position in ORF 278(951)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 10032
Genomic Position 5996860
Position in ORF 825(951)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_70300
Mutant ID PA14NR: 36265
Genomic Position 6269007
Position in ORF 53(951)
Reference 16477005

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PubMed ID: 15687295
Detection of Host-Derived Sphingosine by Pseudomonas aeruginosa Is Important for Survival in the Murine Lung.
Labauve AE, Wargo MJ.
PLoS Pathog. 2014 Jan;10(1):e1003889.
PubMed ID: 24465209
Crystal structure of a COG4313 outer membrane channel.
van den Berg B(1), Bhamidimarri SP(2), Winterhalter M(2).
Sci Rep. 2015 Jul 7;5:11927.
PubMed ID: 26149193