Pseudomonas aeruginosa PAO1, PA5325 (sphA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5325
Name
sphA
Replicon chromosome
Genomic location 5996036 - 5996986 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_254012.1
GI 15600518
Affymetrix PA5325_at
DNASU PaCD00008300
Entrez 880923
GenBank AAG08710.1
INSDC AAG08710.1
NCBI Locus Tag PA5325
protein_id(GenBank) gb|AAG08710.1|AE004945_4|gnl|PseudoCAP|PA5325
TIGR NTL03PA05326
UniParc UPI00000C5FA6
UniProtKB Acc Q9HTN0
UniProtKB ID Q9HTN0_PSEAE
UniRef100 UniRef100_W0Z1I2
UniRef50 UniRef50_Q1I8G0
UniRef90 UniRef90_A6VED2

Product

Feature Type CDS
Coding Frame 1
Product Name
SphA
Product Name Confidence: Class 1
CommentLoss of sphA leads to reduced survival in the murine lung and reduced resistance to the antimicrobial effect of sphingosine.
Evidence for Translation
Charge (pH 7) -7.41
Kyte-Doolittle Hydrophobicity Value -0.263
Molecular Weight (kDa) 35.5
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Class 2
sequence alignment evidence used in manual assertion
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 60 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003078 (1051 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5325
Search term: sphA
Search term: SphA

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 10032
Genomic Position 5996860
Position in ORF 825(951)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 20702
Genomic Position 5996313
Position in ORF 278(951)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Detection of host-derived sphingosine by Pseudomonas aeruginosa is important for survival in the murine lung.
LaBauve AE, Wargo MJ
PLoS Pathog. 2014 Jan;10(1):e1003889
PubMed ID: 24465209
Crystal structure of a COG4313 outer membrane channel.
van den Berg B, Bhamidimarri SP, Winterhalter M
Sci Rep 2015 Jul;5
PubMed ID: 26149193