Pseudomonas aeruginosa PAO1, PA5327 (sphC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5327
Name
sphC
Replicon chromosome
Genomic location 5998348 - 5999676 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254014.1
GI 15600520
Affymetrix PA5327_at
Entrez 878250
GenBank AAG08712.1
INSDC AAG08712.1
NCBI Locus Tag PA5327
protein_id(GenBank) gb|AAG08712.1|AE004945_6|gnl|PseudoCAP|PA5327
TIGR NTL03PA05328
UniParc UPI00000C5FA8
UniProtKB Acc Q9HTM8
UniProtKB ID Q9HTM8_PSEAE
UniRef100 UniRef100_Q9HTM8
UniRef50 UniRef50_Q4KAC2
UniRef90 UniRef90_Q9HTM8

Product

Feature Type CDS
Coding Frame 1
Product Name
SphC
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -2.50
Kyte-Doolittle Hydrophobicity Value -0.276
Molecular Weight (kDa) 49.4
Isoelectric Point (pI) 6.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4218
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 130 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004896 (350 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5327
Search term: sphC
Search term: SphC

Human Homologs

References

Detection of host-derived sphingosine by Pseudomonas aeruginosa is important for survival in the murine lung.
LaBauve AE, Wargo MJ
PLoS Pathog 2014 Jan;10(1):e1003889
PubMed ID: 24465209