Pseudomonas aeruginosa PAO1, PA5328 (sphB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5328
Name
sphB
Replicon chromosome
Genomic location 5999754 - 6000143 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254015.1
GI 15600521
Affymetrix PA5328_at
DNASU PaCD00008301
Entrez 878251
GenBank AAG08713.1
INSDC AAG08713.1
NCBI Locus Tag PA5328
protein_id(GenBank) gb|AAG08713.1|AE004945_7|gnl|PseudoCAP|PA5328
TIGR NTL03PA05329
UniParc UPI00000C5FA9
UniProtKB Acc Q9HTM7
UniProtKB ID Q9HTM7_PSEAE
UniRef100 UniRef100_Q9HTM7
UniRef50 UniRef50_Q4KAC1
UniRef90 UniRef90_Q9HTM7

Product

Feature Type CDS
Coding Frame 1
Product Name
SphB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -1.72
Kyte-Doolittle Hydrophobicity Value -0.046
Molecular Weight (kDa) 13.5
Isoelectric Point (pI) 5.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 96 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004897 (786 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5328
Search term: sphB
Search term: SphB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Detection of host-derived sphingosine by Pseudomonas aeruginosa is important for survival in the murine lung.
LaBauve AE, Wargo MJ
PLoS Pathog 2014 Jan;10(1):e1003889
PubMed ID: 24465209