Pseudomonas aeruginosa PAO1, PA5344 (oxyR)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5344
Name	oxyR
Replicon	chromosome
Genomic location	6012047 - 6012979 (+ strand)
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_254031.1
GI	15600537
Affymetrix	PA5344_at
DNASU	PaCD00008312
Entrez	878254
GenBank	AAG08729.1
INSDC	AAG08729.1
NCBI Locus Tag	PA5344
protein_id(GenBank)	gb|AAG08729.1|AE004946_13|gnl|PseudoCAP|PA5344
TIGR	NTL03PA05345
UniParc	UPI00000C5FB4
UniProtKB Acc	Q9HTL4
UniProtKB ID	Q9HTL4_PSEAE
UniRef100	UniRef100_W0Z0C0
UniRef50	UniRef50_Q4K3Q5
UniRef90	UniRef90_Q4K3Q5

Product

Feature Type	CDS
Coding Frame	1
Product Name	OxyR Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-1.47
Kyte-Doolittle Hydrophobicity Value	-0.023
Molecular Weight (kDa)	34.3
Isoelectric Point (pI)	6.74

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4X6G	DNA BINDING PROTEIN	12/08/14	Full-length OxyR C199D from pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	2	X-RAY DIFFRACTION	99.7
4XWS	DNA BINDING PROTEIN	01/29/15	OxyR regulatory domain C199D mutant from pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	3.006	X-RAY DIFFRACTION	99.6
4Y0M	DNA BINDING PROTEIN	02/06/15	The reduced form of OxyR regulatory domain from Psedomonas aeruginosa	Pseudomonas aeruginosa PAO1	2.3	X-RAY DIFFRACTION	100.0

Virulence Evidence

Source 1
Database	PseudoCAP
Category
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism	Drosophila melanogaster
Notes
Infection model	Drosophila melanogaster killing assay
Reference	15784603
Source 2
Database	PseudoCAP
Category
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism	Mus musculus
Notes
Infection model	Mouse burn Infection model
Reference	15784603
Source 3
Database	PseudoCAP
Category
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism	Homo sapiens
Notes
Infection model	Human neutrophil killing assay
Reference	15784603

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 540 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004912 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5344 Search term: oxyR Search term: OxyR

Human Homologs

References

The Pseudomonas aeruginosa oxidative stress regulator OxyR influences production of pyocyanin and rhamnolipids: protective role of pyocyanin. Vinckx T, Wei Q, Matthijs S, Cornelis P Microbiology (Reading) 2010 Mar;156(Pt 3):678-686 PubMed ID: 19926657

Loss of the oxidative stress regulator OxyR in Pseudomonas aeruginosa PAO1 impairs growth under iron-limited conditions. Vinckx T, Matthijs S, Cornelis P FEMS Microbiol Lett 2008 Nov;288(2):258-65 PubMed ID: 19054085