Pseudomonas aeruginosa PAO1, PA5360 (phoB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5360
Name
phoB
Replicon chromosome
Genomic location 6026779 - 6027468 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254047.1
GI 15600553
Affymetrix PA5360_phoB_at
DNASU PaCD00008323
Entrez 878438
GenBank AAG08745.1
INSDC AAG08745.1
NCBI Locus Tag PA5360
protein_id(GenBank) gb|AAG08745.1|AE004948_1|gnl|PseudoCAP|PA5360
TIGR NTL03PA05361
UniParc UPI00001319B5
UniProtKB Acc P23620
UniProtKB ID PHOB_PSEAE
UniRef100 UniRef100_P23620
UniRef50 UniRef50_P23620
UniRef90 UniRef90_P23620

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component response regulator PhoB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.06
Kyte-Doolittle Hydrophobicity Value -0.236
Molecular Weight (kDa) 25.7
Isoelectric Point (pI) 5.05

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 624 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004925 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5360
Search term: phoB

Human Homologs

References

Phosphate regulation in Pseudomonas aeruginosa: cloning of the alkaline phosphatase gene and identification of phoB- and phoR-like genes.
Filloux A, Bally M, Soscia C, Murgier M, Lazdunski A
Mol Gen Genet 1988 Jun;212(3):510-3
PubMed ID: 3138529