Pseudomonas aeruginosa PAO1, PA5377 (cbcW)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5377
Name
cbcW
Replicon chromosome
Genomic location 6054594 - 6055433 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentCatabolism-associated ABC transporter, interacting with different QAC-specific binding proteins
CommentCatabolism-associated ABC transporter, interacting with different QAC-specific binding proteins

Cross-References

RefSeq NP_254064.1
GI 15600570
Affymetrix PA5377_at
Entrez 878443
GenBank AAG08762.1
INSDC AAG08762.1
NCBI Locus Tag PA5377
protein_id(GenBank) gb|AAG08762.1|AE004950_5|gnl|PseudoCAP|PA5377
TIGR NTL03PA05378
UniParc UPI00000C5FCE
UniProtKB Acc Q9HTI7
UniProtKB ID Q9HTI7_PSEAE
UniRef100 UniRef100_W0YZE5
UniRef50 UniRef50_G8Q8H9
UniRef90 UniRef90_A6VEI8

Product

Feature Type CDS
Coding Frame 1
Product Name
CbcW
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.72
Kyte-Doolittle Hydrophobicity Value 0.967
Molecular Weight (kDa) 30.0
Isoelectric Point (pI) 6.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 451 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004692 (1007 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5377
Search term: cbcW
Search term: CbcW

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis.
Kempf B, Bremer E
J Biol Chem 1995 Jul 14;270(28):16701-13
PubMed ID: 7622480
The ATP-binding cassette transporter Cbc (choline/betaine/carnitine) recruits multiple substrate-binding proteins with strong specificity for distinct quaternary ammonium compounds.
Chen C, Malek AA, Wargo MJ, Hogan DA, Beattie GA
Mol Microbiol 2010 Jan;75(1):29-45
PubMed ID: 19919675