Pseudomonas aeruginosa PAO1, PA5378 (cbcX)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5378
Name	cbcX
Replicon	chromosome
Genomic location	6055475 - 6056413 (- strand)
Transposon Mutants	5 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	binds choline with great affinity. Some betaine binding activity as well.
Comment	Choline (and to a lesser extent, glycine betaine)-specific QAC-binding protein, interacting with the core transporter CbcVW
Comment	Structural similarity to 2REG (ABC-transporter choline binding protein from Rhizobium meliloti, root-mean-square deviation: 0.4 angstroms). Sequence similarity to 2REG (99%/60%) (CHT). Final predicted ligand: choline (PMID: 31631799)
Comment	Choline (and to a lesser extent, glycine betaine)-specific QAC-binding protein, interacting with the core transporter CbcVW

Cross-References

RefSeq	NP_254065.1
GI	15600571
Affymetrix	PA5378_at
Entrez	878444
GenBank	AAG08763.1
INSDC	AAG08763.1
NCBI Locus Tag	PA5378
protein_id(GenBank)	gb|AAG08763.1|AE004950_6|gnl|PseudoCAP|PA5378
TIGR	NTL03PA05379
UniParc	UPI00000C5FCF
UniProtKB Acc	Q9HTI6
UniProtKB ID	Q9HTI6_PSEAE
UniRef100	UniRef100_W0Z1N3
UniRef50	UniRef50_Q2T456
UniRef90	UniRef90_A6VEI9

Product

Feature Type	CDS
Coding Frame	1
Product Name	CbcX Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	0.36
Kyte-Doolittle Hydrophobicity Value	-0.276
Molecular Weight (kDa)	34.4
Isoelectric Point (pI)	7.29

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 142 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004940 (511 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5378 Search term: cbcX Search term: CbcX

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

The ATP-binding cassette transporter Cbc (choline/betaine/carnitine) recruits multiple substrate-binding proteins with strong specificity for distinct quaternary ammonium compounds. Chen C, Malek AA, Wargo MJ, Hogan DA, Beattie GA Mol Microbiol 2010 Jan;75(1):29-45 PubMed ID: 19919675

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799