Pseudomonas aeruginosa PAO1, PA5384 (hocS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5384
Name
hocS
Replicon chromosome
Genomic location 6062352 - 6063347 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 8 transposon mutants in orthologs
CommentDeletion of hocS results in an inability of P. aeruginosa to grow on acetylcarnitine or butyrylcarnitine as sole sources of carbon or nitrogen. Purified HocS hydrolyzes the L-isoforms of acetyl- and butyrylcarnitine.

Cross-References

RefSeq NP_254071.1
GI 15600577
Affymetrix PA5384_at
Entrez 881643
GenBank AAG08769.1
INSDC AAG08769.1
NCBI Locus Tag PA5384
protein_id(GenBank) gb|AAG08769.1|AE004951_3|gnl|PseudoCAP|PA5384
TIGR NTL03PA05385
UniParc UPI00000C5FD5
UniProtKB Acc Q9HTI0
UniProtKB ID Q9HTI0_PSEAE
UniRef100 UniRef100_S0HX90
UniRef50 UniRef50_A6VEJ6
UniRef90 UniRef90_A6VEJ6

Product

Feature Type CDS
Coding Frame 1
Product Name
HocS
Product Name Confidence: Class 1
Synonyms hydrolysis of O-acylcarnitine, short chain
Evidence for Translation
Charge (pH 7) -10.07
Kyte-Doolittle Hydrophobicity Value 0.006
Molecular Weight (kDa) 34.9
Isoelectric Point (pI) 5.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 347 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001165 (2285 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5384
Search term: hocS
Search term: HocS

Human Homologs

Ensembl 110, assembly GRCh38.p14
lipase E, hormone sensitive type [Source:HGNC Symbol;Acc:HGNC:6621]
E-value: 5.5e-15
Percent Identity: 37.1

References

Characterization of Pseudomonas aeruginosa growth on O-acylcarnitines and identification of a short-chain acylcarnitine hydrolase.
Meadows JA, Wargo MJ
Appl Environ Microbiol 2013 Jun;79(11):3355-63
PubMed ID: 23524670