Pseudomonas aeruginosa PAO1, PA5398 (dgcA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5398
Name
dgcA
Synonym: dgcA
Replicon chromosome
Genomic location 6075880 - 6077940 (+ strand)
Transposon Mutants 12 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentStrains with deletions in dgcA are unable to grow on choline, glycine betaine, or dimethylglcycine but are able to utilize sarcosine. NMR analysis shows buildup of labeled dimethylglycine when dgcA mutants are given labeled choline. Presumed to function with DgcB (PA5399).

Cross-References

RefSeq NP_254085.1
GI 15600591
Affymetrix PA5398_at
Entrez 878122
GenBank AAG08783.1
INSDC AAG08783.1
NCBI Locus Tag PA5398
protein_id(GenBank) gb|AAG08783.1|AE004952_5|gnl|PseudoCAP|PA5398
TIGR NTL03PA05399
UniParc UPI00000C5FE3
UniProtKB Acc Q9HTG6
UniProtKB ID Q9HTG6_PSEAE
UniRef100 UniRef100_Q9HTG6
UniRef50 UniRef50_Q9HTG6
UniRef90 UniRef90_Q9HTG6

Product

Feature Type CDS
Coding Frame 1
Product Name
DgcA, Dimethylglycine catabolism
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -13.28
Kyte-Doolittle Hydrophobicity Value -0.216
Molecular Weight (kDa) 75.7
Isoelectric Point (pI) 5.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 509 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004955 (510 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5398
Search term: dgcA

Human Homologs

References

Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
Wargo MJ, Szwergold BS, Hogan DA
J Bacteriol 2008 Apr;190(8):2690-9
PubMed ID: 17951379