Pseudomonas aeruginosa PAO1, PA5497 (nrdJa)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5497
Name	nrdJa
Replicon	chromosome
Genomic location	6188872 - 6191076 (- strand)
Transposon Mutants	3 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	nrdJa and nrdJb are the subunits that form a functional Class II ribonucleotide reductase

Cross-References

RefSeq	NP_254184.1
GI	15600690
Affymetrix	PA5497_at
Entrez	877840
GenBank	AAG08882.1
INSDC	AAG08882.1
NCBI Locus Tag	PA5497
protein_id(GenBank)	gb|AAG08882.1|AE004962_6|gnl|PseudoCAP|PA5497
TIGR	NTL03PA05498
UniParc	UPI00000C6038
UniProtKB Acc	Q9HT76
UniProtKB ID	Q9HT76_PSEAE
UniRef100	UniRef100_Q9HT76
UniRef50	UniRef50_U2E6Q5
UniRef90	UniRef90_A6VEX4

Product

Feature Type	CDS
Coding Frame	1
Product Name	class II (cobalamin-dependent) ribonucleotide-diphosphate reductase subunit, NrdJa Product Name Confidence: Class 1
Synonyms	class II (cobalamin-dependent) ribonucleotide-diphosphate reductase subunit, NrdJa NrdJa (changed from hypothetical protein)
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-10.17
Kyte-Doolittle Hydrophobicity Value	-0.453
Molecular Weight (kDa)	82.7
Isoelectric Point (pI)	5.99

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 579 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG005036 (337 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5497 Search term: nrdJa Search term: class II (cobalamin-dependent) ribonucleotide-diphosphate reductase subunit, NrdJa

Human Homologs

References

Two proteins mediate class II ribonucleotide reductase activity in Pseudomonas aeruginosa: expression and transcriptional analysis of the aerobic enzymes. Torrents E, Poplawski A, Sjöberg BM J Biol Chem 2005 Apr 29;280(17):16571-8 PubMed ID: 15722359