Pseudomonas aeruginosa PA103, PA103_3465 (exoT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Pseudomonas aeruginosa PA103 - Assembly GCF_000611975.2
GCF_000611975.2|latest
Locus Tag
PA103_3465
Name
exoT
Replicon scaffold-98
Genomic location 4331 - 5704 (+ strand)

Cross-References

RefSeq WP_003114583.1
GI 489205537
NCBI Locus Tag PA103_3465

Product

Feature Type CDS
Coding Frame 1
Product Name
exoenzyme T
Product Name Confidence: Class 1
Synonyms ADP-ribosyltransferase
Evidence for Translation
Charge (pH 7) -8.32
Kyte-Doolittle Hydrophobicity Value -0.313
Molecular Weight (kDa) 48.5
Isoelectric Point (pI) 5.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6GNN TOXIN 05/31/18 Exoenzyme T from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, tetrameric crystal form bound to STO1101 Homo sapiens; Pseudomonas aeruginosa PAO1 3.79 X-RAY DIFFRACTION 99.6
6JNP TOXIN 03/17/19 Structure of ExoT-SpcS Complex from Pseudomonas aeruginosa in 2.2 Angstrom Pseudomonas aeruginosa 2.261 X-RAY DIFFRACTION 98.2
4JMF TOXIN/CHAPERONE 03/14/13 Crystal structure of ExoT (residues 28 -77)- SpcS complex from Pseudomonas aeruginosa at 2.1 angstrom Pseudomonas aeruginosa 2.099 X-RAY DIFFRACTION 100.0

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA103_3465
Search term: exoT
Search term: exoenzyme T

Human Homologs

References

ExoT of cytotoxic Pseudomonas aeruginosa prevents uptake by corneal epithelial cells.
Cowell BA, Chen DY, Frank DW, Vallis AJ, Fleiszig SM
Infect Immun 2000 Jan;68(1):403-6
PubMed ID: 10603417