Pseudomonas aeruginosa UCBPP-PA14, PA14_09870 (eftM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_09870
Name
eftM
Replicon chromosome
Genomic location 848003 - 848746 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_788936.1
GI 116052220
Entrez 4382193
INSDC ABJ13456.1
NCBI Locus Tag PA14_09870
UniParc UPI00003962D9
UniProtKB Acc Q02T00
UniProtKB ID Q02T00_PSEAB
UniRef100 UniRef100_Q02T00
UniRef50 UniRef50_Q5E5R1
UniRef90 UniRef90_W0YY28

Product

Feature Type CDS
Coding Frame 1
Product Name
SAM-dependent methyltransferase , EftM
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -4.64
Kyte-Doolittle Hydrophobicity Value -0.357
Molecular Weight (kDa) 28.1
Isoelectric Point (pI) 6.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 139 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001521 (359 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_09870
Search term: eftM

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 37798
Genomic Position 848726
Position in ORF 724(744)
Reference 16477005

Transposon Insertions in Orthologs

Type Confirmed ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4178
Mutant ID UWGC: 3535
Genomic Position 4674225
Position in ORF 263(744)
Reference 14617778
Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4178
Mutant ID UWGC: 21901
Genomic Position 4674168
Position in ORF 206(744)
Reference 14617778

References

Pseudomonas aeruginosa EftM Is a Thermoregulated Methyltransferase.
Owings JP(1), Kuiper EG(2), Prezioso SM(3), Meisner J(4), Varga JJ(1), Zelinskaya N(5), Dammer EB(5), Duong DM(5), Seyfried NT(5), Albertí S(6), Conn GL(5), Goldberg JB(7).
J Biol Chem. 2016 Feb 12;291(7):3280-90.
PubMed ID: 26677219
Lysine trimethylation of EF-Tu mimics platelet-activating factor to initiate Pseudomonas aeruginosa pneumonia.
Barbier M(1), Owings JP, Martínez-Ramos I, Damron FH, Gomila R, Blázquez J, Goldberg JB, Albertí S.
MBio. 2013 May 7;4(3):e00207-13.
PubMed ID: 23653444