Pseudomonas aeruginosa UCBPP-PA14, PA14_09870 (eftM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_09870
Name
eftM
Replicon chromosome
Genomic location 848003 - 848746 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_788936.1
GI 116052220
Entrez 4382193
INSDC ABJ13456.1
NCBI Locus Tag PA14_09870
UniParc UPI00003962D9
UniProtKB Acc Q02T00
UniProtKB ID Q02T00_PSEAB
UniRef100 UniRef100_Q02T00
UniRef50 UniRef50_Q5E5R1
UniRef90 UniRef90_W0YY28

Product

Feature Type CDS
Coding Frame 1
Product Name
SAM-dependent methyltransferase , EftM
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -4.64
Kyte-Doolittle Hydrophobicity Value -0.357
Molecular Weight (kDa) 28.1
Isoelectric Point (pI) 6.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 139 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001521 (354 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_09870
Search term: eftM

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 37798
Genomic Position 848726
Position in ORF 724(744)
Reference 16477005

Transposon Insertions in Orthologs

Type Confirmed ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4178
Mutant ID UWGC: 3535
Genomic Position 4674225
Position in ORF 263(744)
Reference 14617778
Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4178
Mutant ID UWGC: 21901
Genomic Position 4674168
Position in ORF 206(744)
Reference 14617778

References

Pseudomonas aeruginosa EftM Is a Thermoregulated Methyltransferase.
Owings JP, Kuiper EG, Prezioso SM, Meisner J, Varga JJ, Zelinskaya N, Dammer EB, Duong DM, Seyfried NT, Albertí S, Conn GL, Goldberg JB
J. Biol. Chem. 2016 Feb;291(7):3280-90
PubMed ID: 26677219
Lysine trimethylation of EF-Tu mimics platelet-activating factor to initiate Pseudomonas aeruginosa pneumonia.
Barbier M, Owings JP, Martínez-Ramos I, Damron FH, Gomila R, Blázquez J, Goldberg JB, Albertí S
MBio 2013 May;4(3):e00207-13
PubMed ID: 23653444