Pseudomonas aeruginosa UCBPP-PA14, PA14_13660 (tpbA)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
Replicon chromosome
Genomic location 1174167 - 1174823 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq YP_789236.1
GI 116051921
Entrez 4381929
INSDC ABJ13157.1
NCBI Locus Tag PA14_13660
UniParc UPI00003961FB
UniProtKB Acc Q02S50
UniRef100 UniRef100_Q02S50
UniRef50 UniRef50_Q9HXC7
UniRef90 UniRef90_Q9HXC7


Feature Type CDS
Coding Frame 1
Product Name
protein tyrosine phosphatase TpbA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) 1.34
Kyte-Doolittle Hydrophobicity Value -0.121
Molecular Weight (kDa) 24.0
Isoelectric Point (pI) 7.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
cell fractionation evidence
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R0T Hydrolase 08/01/14 Crystal structure of P. aeruginosa TpbA (C132S) in complex with pTyr PSEUDOMONAS AERUGINOSA PAO1 2.603 X-RAY DIFFRACTION 97.7
4R0S Hydrolase 08/01/14 Crystal structure of P. aeruginosa TpbA PSEUDOMONAS AERUGINOSA PAO1 2.03 X-RAY DIFFRACTION 98.2
2M3V HYDROLASE 01/26/13 Solution structure of tyrosine phosphatase related to biofilm formation A (TpbA) from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 99.5

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 32 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001786 (431 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA14_13660
Search term: tpbA

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 25687
Genomic Position 1174750
Position in ORF 584(657)
Reference 16477005

Transposon Insertions in Orthologs

Type ISphoA/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA3885
Mutant ID UWGC: 35319
Genomic Position 4351288
Position in ORF 455(657)
Reference 14617778


Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885).
Ueda A, Wood TK.
PLoS Pathog. 2009 Jun;5(6):e1000483.
PubMed ID: 19543378