Pseudomonas aeruginosa UCBPP-PA14, PA14_13660 (tpbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_13660
Name
tpbA
Replicon chromosome
Genomic location 1174167 - 1174823 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq YP_789236.1
GI 116051921
Entrez 4381929
INSDC ABJ13157.1
NCBI Locus Tag PA14_13660
UniParc UPI00003961FB
UniProtKB Acc Q02S50
UniProtKB ID Q02S50_PSEAB
UniRef100 UniRef100_Q02S50
UniRef50 UniRef50_Q9HXC7
UniRef90 UniRef90_Q9HXC7

Product

Feature Type CDS
Coding Frame 1
Product Name
protein tyrosine phosphatase TpbA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) 1.34
Kyte-Doolittle Hydrophobicity Value -0.121
Molecular Weight (kDa) 24.0
Isoelectric Point (pI) 7.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
cell fractionation evidence
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R0T Hydrolase 08/01/14 Crystal structure of P. aeruginosa TpbA (C132S) in complex with pTyr PSEUDOMONAS AERUGINOSA PAO1 2.603 X-RAY DIFFRACTION 97.7
4R0S Hydrolase 08/01/14 Crystal structure of P. aeruginosa TpbA PSEUDOMONAS AERUGINOSA PAO1 2.03 X-RAY DIFFRACTION 98.2
2M3V HYDROLASE 01/26/13 Solution structure of tyrosine phosphatase related to biofilm formation A (TpbA) from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 99.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 32 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001786 (431 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_13660
Search term: tpbA

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 25687
Genomic Position 1174750
Position in ORF 584(657)
Reference 16477005

Transposon Insertions in Orthologs

Type ISphoA/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA3885
Mutant ID UWGC: 35319
Genomic Position 4351288
Position in ORF 455(657)
Reference 14617778

References

Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885).
Ueda A, Wood TK.
PLoS Pathog. 2009 Jun;5(6):e1000483.
PubMed ID: 19543378