Pseudomonas aeruginosa UCBPP-PA14, PA14_43100 (rhsP2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_43100
Name
rhsP2
Replicon chromosome
Genomic location 3835369 - 3840213 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
CommentHcp Secretion Island-II-encoded type VI secretion system (H2-T6SS)

Cross-References

RefSeq YP_791594.1
GI 116049601
Entrez 4381809
INSDC ABJ10837.1
NCBI Locus Tag PA14_43100
UniParc UPI000045D1A9
UniProtKB Acc Q02KE2
UniProtKB ID Q02KE2_PSEAB
UniRef100 UniRef100_S0J0Y5
UniRef50 UniRef50_U7DIR8
UniRef90 UniRef90_X6XLE7

Product

Feature Type CDS
Coding Frame 1
Product Name
RhsP2
Product Name Confidence: Class 1
Synonyms Rhs family protein
Evidence for Translation
Charge (pH 7) -20.06
Kyte-Doolittle Hydrophobicity Value -0.596
Molecular Weight (kDa) 182.2
Isoelectric Point (pI) 6.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7RT7 TOXIN/ANTITOXIN 08/12/21 Crystal structure of the RhsP2 C-terminal toxin domain in complex with its immunity protein, RhsI2 Pseudomonas aeruginosa (strain UCBPP-PA14) 2.49 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration model
Reference 19497948
Source 2
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse acute lung Infection model
Infection model Mouse burn Infection model
Reference 19497948

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 213 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG014915 (106 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_43100
Search term: rhsP2
Search term: RhsP2

Human Homologs

References

An rhs gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14.
Jones C, Hachani A, Manoli E, Filloux A
J Bacteriol 2014 Feb;196(4):800-10
PubMed ID: 24317402
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A
Proc Natl Acad Sci U S A 2017 Jul 18;114(29):7707-7712
PubMed ID: 28673999