Pseudomonas aeruginosa UCBPP-PA14, PA14_49890 (tpbB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_49890
Name
tpbB
Replicon chromosome
Genomic location 4435431 - 4436738 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq YP_792147.1
GI 116049051
Entrez 4380087
INSDC ABJ10287.1
NCBI Locus Tag PA14_49890
UniParc UPI000045D0B2
UniProtKB Acc Q02IT9
UniProtKB ID Q02IT9_PSEAB
UniRef100 UniRef100_Q02IT9
UniRef50 UniRef50_G8Q662
UniRef90 UniRef90_W0YL25

Product

Feature Type CDS
Coding Frame 1
Product Name
diguanylate cyclase TpbB
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -4.88
Kyte-Doolittle Hydrophobicity Value -0.077
Molecular Weight (kDa) 47.5
Isoelectric Point (pI) 6.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4IOB LYASE 01/07/13 Crystal structure of the GGDEF domain of PA1120 (YfiN or TpbB) from Pseudomonas aeruginosa at 2.7 Ang. PSEUDOMONAS AERUGINOSA 2.78 X-RAY DIFFRACTION 99.4

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 456 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001080 (471 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_49890
Search term: tpbB

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 37761
Genomic Position 4435614
Position in ORF 184(1308)
Reference 16477005

Transposon Insertions in Orthologs

Type Confirmed ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA1120
Mutant ID UWGC: 8312
Genomic Position 1214199
Position in ORF 1005(1308)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA1120
Mutant ID UWGC: 43263
Genomic Position 1213299
Position in ORF 105(1308)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA1120
Mutant ID UWGC: 8312
Genomic Position 1214199
Position in ORF 1005(1308)
Reference 14617778

References

Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885).
Ueda A, Wood TK.
PLoS Pathog. 2009 Jun;5(6):e1000483.
PubMed ID: 19543378