Pseudomonas aeruginosa UCBPP-PA14, PA14_51340 (mvfR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_51340
Name
mvfR
Replicon chromosome
Genomic location 4562837 - 4563835 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq YP_792268.1
GI 116048931
Entrez 4379987
INSDC ABJ10167.1
NCBI Locus Tag PA14_51340
UniParc UPI0000E44B70
UniProtKB Acc Q02IG8
UniProtKB ID Q02IG8_PSEAB
UniRef100 UniRef100_Q02IG8
UniRef50 UniRef50_Q02IG8
UniRef90 UniRef90_Q02IG8

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator MvfR
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 1.77
Kyte-Doolittle Hydrophobicity Value -0.131
Molecular Weight (kDa) 37.2
Isoelectric Point (pI) 7.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Membrane Class 1
cell fractionation evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7QAV GENE REGULATION 11/17/21 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with compound N-((2-(4-cyclopropylphenyl)thiazol-5-yl)methyl)-2-(trifluoromethyl)pyridin-4-amine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.65 X-RAY DIFFRACTION 99.4
6YIZ DNA BINDING PROTEIN 04/01/20 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with triazolo-pyridine inverse agonist A Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.163 X-RAY DIFFRACTION 99.6
4JVC TRANSCRIPTION REGULATOR 03/25/13 Crystal structure of PqsR co-inducer binding domain Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 100.0
7O2U DNA BINDING PROTEIN 03/31/21 PqsR (MvfR) in complex with antagonist 40 Pseudomonas aeruginosa 3 X-RAY DIFFRACTION 100.0
6Q7U DNA BINDING PROTEIN 12/13/18 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with HHQ Pseudomonas aeruginosa PAO1 3.14 X-RAY DIFFRACTION 99.6
6TPR DNA BINDING PROTEIN 12/14/19 PqsR (MvfR) bound to inhibitory compound 40 Pseudomonas aeruginosa UCBPP-PA14 3.2 X-RAY DIFFRACTION 100.0
7QA0 GENE REGULATION 11/15/21 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with compound 1456 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.67 X-RAY DIFFRACTION 99.7
6B8A TRANSCRIPTION 10/05/17 Crystal structure of MvfR ligand binding domain in complex with M64 Pseudomonas aeruginosa 2.65 X-RAY DIFFRACTION 100.0
7O2T DNA BINDING PROTEIN 03/31/21 PqsR (MvfR) in complex with antagonist 61 Pseudomonas aeruginosa 2.65 X-RAY DIFFRACTION 100.0
6Q7V DNA BINDING PROTEIN 12/13/18 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with compound 11 Pseudomonas aeruginosa PAO1 2.56 X-RAY DIFFRACTION 99.6
6Z17 DNA BINDING PROTEIN 05/12/20 PqsR (MvfR) in complex with antagonist 6 Pseudomonas aeruginosa (strain UCBPP-PA14) 3.15 X-RAY DIFFRACTION 100.0
6Z07 DNA BINDING PROTEIN 05/07/20 PqsR (MvfR) in complex with antagonist 12 Pseudomonas aeruginosa (strain UCBPP-PA14) 2.95 X-RAY DIFFRACTION 100.0
4JVD TRANSCRIPTION REGULATOR 03/25/13 Crystal structure of PqsR coinducer binding domain of Pseudomonas aeruginosa with ligand NHQ Pseudomonas aeruginosa 2.95 X-RAY DIFFRACTION 100.0
4JVI TRANSCRIPTION REGULATOR/INHIBITOR 03/25/13 Crystal structure of PqsR co-inducer binding domain of Pseudomonas aeruginosa with inhibitor 3NH2-7Cl-C9QZN Pseudomonas aeruginosa 2.9 X-RAY DIFFRACTION 100.0
6YZ3 DNA BINDING PROTEIN 05/06/20 PqsR (MvfR) in complex with antagonist 19 Pseudomonas aeruginosa 3 X-RAY DIFFRACTION 100.0
7NBW DNA BINDING PROTEIN 01/28/21 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with a pyridin agonist Pseudomonas aeruginosa PAO1 2.28 X-RAY DIFFRACTION 99.6
6Z5K DNA BINDING PROTEIN 05/26/20 PqsR (MvfR) in complex with antagonist 18 Pseudomonas aeruginosa (strain UCBPP-PA14) 3.201 X-RAY DIFFRACTION 100.0
6Q7W DNA BINDING PROTEIN 12/13/18 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with compound 20 Pseudomonas aeruginosa PAO1 2.82 X-RAY DIFFRACTION 99.6
7QA3 GENE REGULATION 11/15/21 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with compound N-((2-(4-phenoxyphenyl)thiazol-5-yl)methyl)-2-(trifluoromethyl)pyridin-4-amine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.67 X-RAY DIFFRACTION 99.7
7P4U GENE REGULATION 07/13/21 Crystal structure of PqsR (MvfR) ligand-binding domain in complex with 3-PYRIDIN-4-YL-2,4-DIHYDRO-INDENO[1,2-.C.]PYRAZOLE Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.74 X-RAY DIFFRACTION 99.6

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse thermal injury model.
Reference 11724939
Source 2
Database Victors : 4399
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Reference 9371831
Source 3
Database PseudoCAP
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration assay
Reference 11724939
Source 4
Database PseudoCAP
Category Phenazine biosynthesis
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model acute pulmonary Infection model
Infection model chronic pulmonary Infection model
Reference 15213173

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 139 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000966 (291 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_51340
Search term: mvfR

Human Homologs

References

A quorum sensing-associated virulence gene of Pseudomonas aeruginosa encodes a LysR-like transcription regulator with a unique self-regulatory mechanism.
Cao H, Krishnan G, Goumnerov B, Tsongalis J, Tompkins R, Rahme LG
Proc Natl Acad Sci U S A 2001 Dec 4;98(25):14613-8
PubMed ID: 11724939