Pseudomonas aeruginosa UCBPP-PA14, PA14_61840

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_61840
Name
Replicon chromosome
Genomic location 5514196 - 5514516 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14

Cross-References

RefSeq YP_793136.1
GI 116052819
Entrez 4385672
INSDC ABJ14055.1
NCBI Locus Tag PA14_61840
UniParc UPI0000E44C22
UniProtKB Acc Q02G00
UniProtKB ID Q02G00_PSEAB
UniRef100 UniRef100_S0J386
UniRef50 UniRef50_A6VCC3
UniRef90 UniRef90_A6VCC3

Product

Feature Type CDS
Coding Frame 1
Product Name
Antitoxin HigA
Product Name Confidence: Class 1
Synonyms virulence-associated protein
Evidence for Translation
Charge (pH 7) -5.29
Kyte-Doolittle Hydrophobicity Value -0.150
Molecular Weight (kDa) 11.2
Isoelectric Point (pI) 4.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7CSY ANTITOXIN/DNA 08/17/20 Pseudomonas aeruginosa antitoxin HigA with higBA promoter Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa UCBPP-PA14 2.29 X-RAY DIFFRACTION 98.0
7CSV ANTITOXIN 08/17/20 Pseudomonas aeruginosa antitoxin HigA Pseudomonas aeruginosa PAO1 1.71 X-RAY DIFFRACTION 98.0
6JPI ANTITOXIN/DNA 03/27/19 Crystal structure of PA4674 in complex with its operator DNA (28bp) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.143 X-RAY DIFFRACTION 98.0
6LB3 ANTITOXIN/DNA 11/13/19 Crystal structure of PA4674 in complex with its operator DNA (18bp) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 2.497 X-RAY DIFFRACTION 98.0
7CSW ANTITOXIN/DNA 08/17/20 Pseudomonas aeruginosa antitoxin HigA with pa2440 promoter Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa UCBPP-PA14 1.97 X-RAY DIFFRACTION 98.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 219 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004324 (343 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_61840
Search term: Antitoxin HigA

Human Homologs

References

The HigB/HigA toxin/antitoxin system of Pseudomonas aeruginosa influences the virulence factors pyochelin, pyocyanin, and biofilm formation.
Wood TL, Wood TK
Microbiologyopen 2016 Jun;5(3):499-511
PubMed ID: 26987441