Pseudomonas aeruginosa UCBPP-PA14, PA14_70200 (dppA5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_70200
Name
dppA5
Replicon chromosome
Genomic location 6259725 - 6261305 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq YP_793791.1
GI 116053464
Entrez 4384019
INSDC ABJ14700.1
NCBI Locus Tag PA14_70200
UniParc UPI0000E44C7B
UniProtKB Acc Q02E45
UniProtKB ID Q02E45_PSEAB
UniRef100 UniRef100_W1MWW8
UniRef50 UniRef50_W6R3H6
UniRef90 UniRef90_A6VEC4

Product

Feature Type CDS
Coding Frame 1
Product Name
dipeptide ABC transporter substrate-binding protein DppA5
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -0.22
Kyte-Doolittle Hydrophobicity Value -0.233
Molecular Weight (kDa) 57.9
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 550 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004888 (540 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_70200
Search term: dppA5

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 23775
Genomic Position 6259842
Position in ORF 118(1581)
Reference 16477005

Transposon Insertions in Orthologs

Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5317
Mutant ID UBC: PAO1_lux_52_E12
Genomic Position 5987659
Position in ORF 1185(1581)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5317
Mutant ID UWGC: 8853
Genomic Position 5987828
Position in ORF 1354(1581)
Reference 14617778
Type ISphoA/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5317
Mutant ID UWGC: 30472
Genomic Position 5986822
Position in ORF 348(1581)
Reference 14617778
Type ISphoA/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5317
Mutant ID UWGC: 32922
Genomic Position 5987377
Position in ORF 903(1581)
Reference 14617778

References

High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D(1), Lafon C(2), Braun Y(1), Köhler T(3), Page MG(4), Mourez M(2), Weingart H(1).
PLoS One. 2014 Oct 22;9(10):e111311.
PubMed ID: 25338022