Pseudomonas aeruginosa UCBPP-PA14, PA14_70300 (sphA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_70300
Name
sphA
Replicon chromosome
Genomic location 6268955 - 6269905 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_793799.1
GI 116053472
Entrez 4385088
INSDC ABJ14708.1
NCBI Locus Tag PA14_70300
UniParc UPI0000397091
UniProtKB Acc Q02E37
UniProtKB ID Q02E37_PSEAB
UniRef100 UniRef100_S0HIE1
UniRef50 UniRef50_Q1I8G0
UniRef90 UniRef90_A6VED2

Product

Feature Type CDS
Coding Frame 1
Product Name
SphA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -7.41
Kyte-Doolittle Hydrophobicity Value -0.289
Molecular Weight (kDa) 35.6
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Class 2
sequence alignment evidence used in manual assertion
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 60 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003078 (1061 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_70300
Search term: sphA
Search term: SphA

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 36265
Genomic Position 6269007
Position in ORF 53(951)
Reference 16477005

Transposon Insertions in Orthologs

Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5325
Mutant ID UWGC: 10032
Genomic Position 5996860
Position in ORF 825(951)
Reference 14617778
Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5325
Mutant ID UWGC: 20702
Genomic Position 5996313
Position in ORF 278(951)
Reference 14617778

References

Crystal structure of a COG4313 outer membrane channel.
van den Berg B(1), Bhamidimarri SP(2), Winterhalter M(2).
Sci Rep. 2015 Jul 7;5:11927.
PubMed ID: 26149193
Detection of Host-Derived Sphingosine by Pseudomonas aeruginosa Is Important for Survival in the Murine Lung.
Labauve AE, Wargo MJ.
PLoS Pathog. 2014 Jan;10(1):e1003889.
PubMed ID: 24465209