Pseudomonas aeruginosa UCBPP-PA14, PA14_70300 (sphA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_70300
Name
sphA
Replicon chromosome
Genomic location 6268955 - 6269905 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_793799.1
GI 116053472
Entrez 4385088
INSDC ABJ14708.1
NCBI Locus Tag PA14_70300
UniParc UPI0000397091
UniProtKB Acc Q02E37
UniProtKB ID Q02E37_PSEAB
UniRef100 UniRef100_S0HIE1
UniRef50 UniRef50_Q1I8G0
UniRef90 UniRef90_A6VED2

Product

Feature Type CDS
Coding Frame 1
Product Name
SphA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -7.41
Kyte-Doolittle Hydrophobicity Value -0.289
Molecular Weight (kDa) 35.6
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 2
sequence alignment evidence used in manual assertion
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 60 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003078 (1061 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_70300
Search term: sphA
Search term: SphA

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 36265
Genomic Position 6269007
Position in ORF 53(951)
Reference 16477005

Transposon Insertions in Orthologs

Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5325
Mutant ID UWGC: 10032
Genomic Position 5996860
Position in ORF 825(951)
Reference 14617778
Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5325
Mutant ID UWGC: 20702
Genomic Position 5996313
Position in ORF 278(951)
Reference 14617778

References

Crystal structure of a COG4313 outer membrane channel.
van den Berg B(1), Bhamidimarri SP(2), Winterhalter M(2).
Sci Rep. 2015 Jul 7;5:11927.
PubMed ID: 26149193
Detection of Host-Derived Sphingosine by Pseudomonas aeruginosa Is Important for Survival in the Murine Lung.
Labauve AE, Wargo MJ.
PLoS Pathog. 2014 Jan;10(1):e1003889.
PubMed ID: 24465209