Pseudomonas aeruginosa UCBPP-PA14, PA14_70310 (sphD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_70310
Name
sphD
Replicon chromosome
Genomic location 6269976 - 6271184 (- strand)
Transposon Mutants 3 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_793800.1
GI 116053473
Entrez 4385089
INSDC ABJ14709.1
NCBI Locus Tag PA14_70310
UniParc UPI0000E44C7E
UniProtKB Acc Q02E36
UniProtKB ID Q02E36_PSEAB
UniRef100 UniRef100_S0HZY4
UniRef50 UniRef50_A6VED3
UniRef90 UniRef90_A6VED3

Product

Feature Type CDS
Coding Frame 1
Product Name
SphD
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) 3.86
Kyte-Doolittle Hydrophobicity Value -0.137
Molecular Weight (kDa) 44.5
Isoelectric Point (pI) 8.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 30 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004895 (363 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_70310
Search term: sphD
Search term: SphD

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 31461
Genomic Position 6270433
Position in ORF 458(1209)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 45507
Genomic Position 6270520
Position in ORF 545(1209)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 25366
Genomic Position 6271157
Position in ORF 1182(1209)
Reference 16477005

Transposon Insertions in Orthologs

Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5326
Mutant ID UWGC: 10484
Genomic Position 5997560
Position in ORF 504(1209)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA5326
Mutant ID UWGC: 9185
Genomic Position 5997552
Position in ORF 496(1209)
Reference 14617778

References

Detection of Host-Derived Sphingosine by Pseudomonas aeruginosa Is Important for Survival in the Murine Lung.
Labauve AE, Wargo MJ.
PLoS Pathog. 2014 Jan;10(1):e1003889.
PubMed ID: 24465209