Pseudomonas aeruginosa PA7, PSPA7_2375 (pumA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PA7
Locus Tag
PSPA7_2375
Name
pumA
Replicon chromosome
Genomic location 2420133 - 2421044 (+ strand)

Cross-References

RefSeq YP_001347742.1
GI 152988748
Entrez 5358979
INSDC ABR85931.1
NCBI Locus Tag PSPA7_2375
UniParc UPI000157754F
UniProtKB Acc A6V3V7
UniProtKB ID A6V3V7_PSEA7
UniRef100 UniRef100_A6V3V7
UniRef50 UniRef50_A6V3V7
UniRef90 UniRef90_A6V3V7

Product

Feature Type CDS
Coding Frame 1
Product Name
PumA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -9.18
Kyte-Doolittle Hydrophobicity Value -0.296
Molecular Weight (kDa) 34642.3
Isoelectric Point (pI) 4.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Homo sapiens
Notes PumA is essential for control of NF-?B translocation into the nucleus during Pseudomonas aeruginosa infection of human A549 lung epithelial cells
Infection model Infection of human A549 lung carcinoma epithelial cells
Reference 28483816
Source 2
Database PseudoCAP
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes PA7 pumA mutant-infected mice showed decreased cell recruitment and an enhanced lung bacterial clearance in bronchoalveolar lavages compared to the wild-type strain.
Infection model Mouse pulmonary infection model
Reference 28483816
Source 3
Database PseudoCAP
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Caenorhabditis elegans
Notes PA7 wild-type strain caused 50% lethality 7 days after inoculation while PA7 pumA mutant showed a slight but significant attenuation in virulence in C. elegans.
Infection model C. elegans slow killing assay.
Reference 28483816

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 56 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG024070 (9 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PSPA7_2375
Search term: pumA
Search term: PumA

References

PubMed ID: 28483816