Pseudomonas entomophila L48, PSEEN3174
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas entomophila L48
GCF_000026105.1|latest |
| Locus Tag |
PSEEN3174
|
| Name |
|
| Replicon | chromosome |
| Genomic location | 3389878 - 3390693 (- strand) |
| Comment | Monalysin is a pore-forming toxin that contributes to the virulence of P. entomophila against Drosophila. Monalysin requires N-terminal cleavage to become fully active, forms oligomers in vitro, and induces pore formation in artificial lipid membranes. |
Cross-References
| RefSeq | YP_608728.1 |
| GI | 104782230 |
| Entrez | 4088838 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
monalysin
|
| Synonyms |
hypothetical protein |
| Evidence for Translation | |
| Charge (pH 7) | -0.84 |
| Kyte-Doolittle Hydrophobicity Value | -0.452 |
| Molecular Weight (kDa) | 30201.2 |
| Isoelectric Point (pI) | 6.68 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4MKQ | TOXIN | 09/05/13 | Crystal structure of the Pore-Forming Toxin Monalysin mutant deleted of the membrane-spanning domain | Pseudomonas entomophila | 2.65 | X-RAY DIFFRACTION | 100.0 |
| 4MJT | TOXIN | 09/04/13 | Crystal structure of the oligomeric pore-forming toxin pro-Monalysin | Pseudomonas entomophila | 2.85 | X-RAY DIFFRACTION | 100.0 |
| 4MKO | TOXIN | 09/05/13 | Crystal structure of the monomeric, cleaved form of the Pore-Forming Toxin Monalysin | Pseudomonas entomophila | 1.7 | X-RAY DIFFRACTION | 100.0 |
Virulence Evidence
| Source 1 | |
| Database | PseudoCAP |
| Category | |
| Evidence |
ECO:0000314
direct assay evidence used in manual assertion
|
| Host Organism | Drosophila melanogaster |
| Notes | Reduced cell death iobserved upon infection with a mutant deficient in Monalysin. Monalysin plays a role in P. entomophila ability to induce intestinal cell damage. |
| Reference | 21980286 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG023162 (5 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PSEEN3174
Search term: monalysin
|
Human Homologs
References
|
Monalysin, a novel ß-pore-forming toxin from the Drosophila pathogen Pseudomonas entomophila, contributes to host intestinal damage and lethality.
Opota O, Vallet-Gély I, Vincentelli R, Kellenberger C, Iacovache I, Gonzalez MR, Roussel A, van der Goot FG, Lemaitre B
PLoS Pathog 2011 Sep;7(9):e1002259
PubMed ID: 21980286
|