Pseudomonas entomophila L48, PSEEN3174

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas entomophila L48
Locus Tag
Replicon chromosome
Genomic location 3389878 - 3390693 (- strand)


RefSeq YP_608728.1
GI 104782230
Entrez 4088838


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
CommentMonalysin is a pore-forming toxin that contributes to the virulence of P. entomophila against Drosophila. Monalysin requires N-terminal cleavage to become fully active, forms oligomers in vitro, and induces pore formation in artificial lipid membranes.
Evidence for Translation
Charge (pH 7) -0.84
Kyte-Doolittle Hydrophobicity Value -0.452
Molecular Weight (kDa) 30201.2
Isoelectric Point (pI) 6.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4MJT TOXIN 09/04/13 Crystal structure of the oligomeric pore-forming toxin pro-Monalysin PSEUDOMONAS ENTOMOPHILA 2.85 X-RAY DIFFRACTION 100.0
4MKO TOXIN 09/05/13 Crystal structure of the monomeric, cleaved form of the Pore-Forming Toxin Monalysin PSEUDOMONAS ENTOMOPHILA 1.7 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Drosophila melanogaster
Notes Reduced cell death iobserved upon infection with a mutant deficient in Monalysin. Monalysin plays a role in P. entomophila ability to induce intestinal cell damage.
Reference 21980286

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG023162 (6 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PSEEN3174
Search term: monalysin


Monalysin, a novel ß-pore-forming toxin from the Drosophila pathogen Pseudomonas entomophila, contributes to host intestinal damage and lethality.
Opota O, Vallet-Gély I, Vincentelli R, Kellenberger C, Iacovache I, Gonzalez MR, Roussel A, van der Goot FG, Lemaitre B
PLoS Pathog. 2011 Sep;7(9):e1002259
PubMed ID: 21980286