Pseudomonas aeruginosa PAO1, PA5183.1 (rsmN)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA5183.1
|
| NCBI Locus Tag | PA5183a |
| Name |
rsmN
|
| Replicon | chromosome |
| Genomic location | 5836470 - 5836685 (- strand) |
| Comment | Post-transcriptional riboregulator |
| Comment | Post-transcriptional riboregulator |
Cross-References
| RefSeq | YP_008719784.1 |
| GI | |
| Entrez | 17373387 |
| NCBI Locus Tag | PA5183a |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
RsmN
|
| Synonyms |
RNA-binding global regulator CsrA Uncharacterized protein |
| Evidence for Translation |
Translation confirmed using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | 0.99 |
| Kyte-Doolittle Hydrophobicity Value | -0.020 |
| Molecular Weight (kDa) | 7.8 |
| Isoelectric Point (pI) | 9.21 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4KJI | RNA BINDING PROTEIN/RNA | 05/03/13 | Novel re-arrangement of an RsmA/cSRa family protein to create a structurally distinct new RNA-binding family member | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 3.2 | X-RAY DIFFRACTION | 100.0 |
| 4K59 | RNA BINDING PROTEIN | 04/14/13 | Crystal Structure of Pseudomonas Aeruginosa RsmF | Pseudomonas aeruginosa | 2.22 | X-RAY DIFFRACTION | 100.0 |
| 4KRW | RNA BINDING PROTEIN | 05/17/13 | Novel re-arrangement of an RsmA/cSRa family protein to create a structurally distinct new RNA-binding family member | Pseudomonas aeruginosa | 2.01 | X-RAY DIFFRACTION | 100.0 |
Pathogen Association Analysis
| Results | Analysis not done |
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG008415 (310 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA5183.1
Search term: rsmN
Search term: RsmN
|
Human Homologs
References
|
Structural rearrangement in an RsmA/CsrA ortholog of Pseudomonas aeruginosa creates a dimeric RNA-binding protein, RsmN.
Morris ER, Hall G, Li C, Heeb S, Kulkarni RV, Lovelock L, Silistre H, Messina M, Cámara M, Emsley J, Williams P, Searle MS
Structure 2013 Sep 3;21(9):1659-71
PubMed ID: 23954502
|
|
LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan 16;96:328-342
PubMed ID: 24291602
|