Pseudomonas aeruginosa LESB58, PALES_21291 (cif)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa LESB58
GCF_000026645.1|latest |
| Locus Tag |
PALES_21291
|
| Name |
cif
|
| Replicon | chromosome |
| Genomic location | 2304477 - 2305436 (- strand) |
Cross-References
| RefSeq | |
| GI | 218890868 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
CFTR inhibitory factor, Cif
|
| Synonyms | |
| Evidence for Translation | |
| Charge (pH 7) | -3.21 |
| Kyte-Doolittle Hydrophobicity Value | -0.149 |
| Molecular Weight (kDa) | 35.8 |
| Isoelectric Point (pI) | 6.51 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 5HKB | Hydrolase/Hydrolase inhibitor | 01/13/16 | Crystal structure of the CFTR inhibitory factor Cif bound to the inhibitor KB2115 | Pseudomonas aeruginosa UCBPP-PA14 | 1.65 | X-RAY DIFFRACTION | 100.0 |
| 5HK9 | HYDROLASE/HYDROLASE INHIBITOR | 01/13/16 | Crystal structure of the CFTR inhibitory factor Cif bound to a urea inhibitor | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.8 | X-RAY DIFFRACTION | 100.0 |
| 4YX9 | Hydrolase/Hydrolase inhibitor | 03/22/15 | Crystal structure of the CFTR inhibitory factor Cif bound to tiratricol | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.75 | X-RAY DIFFRACTION | 100.0 |
| 5TNM | HYDROLASE | 10/14/16 | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted (R)-1,2-Epoxyoctane hydrolysis intermediate | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.7 | X-RAY DIFFRACTION | 99.7 |
| 3KD2 | HYDROLASE | 10/22/09 | Crystal structure of the CFTR inhibitory factor Cif | Pseudomonas aeruginosa UCBPP-PA14 | 1.8 | X-RAY DIFFRACTION | 100.0 |
| 4EUS | HYDROLASE | 04/25/12 | Crystal structure of the CFTR inhibitory factor Cif bound to 1,2-hexanediol | Pseudomonas aeruginosa | 1.65 | X-RAY DIFFRACTION | 100.0 |
| 5HKA | Hydrolase/Hydrolase inhibitor | 01/13/16 | Crystal structure of the CFTR inhibitory factor Cif bound to an amide inhibitor | Pseudomonas aeruginosa (strain UCBPP-PA14) | 2.05 | X-RAY DIFFRACTION | 100.0 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 308 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000798 (2691 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PALES_21291
Search term: cif
Search term: CFTR inhibitory factor, Cif
|
Human Homologs
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
References
|
Cif is negatively regulated by the TetR family repressor CifR.
MacEachran DP, Stanton BA, O'Toole GA
Infect Immun 2008 Jul;76(7):3197-206
PubMed ID: 18458065
|