May 12, 2005
New gene name: plcB. New protein name: phospholipase C, PlcB. New protein name confidence: Class 1. New subcellular localization: Extracellular. New subcellular localization confidence: Class 1. New evidence reference: 15306013. New structure feature: 3 His residues are involved in coordinating three zinc ions in the active site. The region encompassing the active site shares limited, but significant similarity to the zinc dependent PlCs of Gram-positive bacteria, including Bacillus cereus, Listeria monocytogenes (PlcB), Clostridium perfringens (alpha toxin). New genomic context: PA0026, PA0027 & PA0028 constitute an operon. New comment: PlcB is required for directed twitching motility up a gradient of phosphatidylcholine (PC), or phosphatidylethanolamine (PE) with specific type of fatty acid residues. PlcB can hyrolyse both PC and PE, and secreted via the Sec pathway. Proteins encoded by PA0027 & PA0028 are required for the proper folding (PA0027) of PlcB and to chaperone (PA0028) it through the inner and outer membrane.
Genevieve Ball, Adam P. Parker, Fiona S.L. Brinkman, Alain Filloux, Raymond C. Lo., Michael L. Vasil, Adriana I. Vasil, Paula J. Wilderman