Pseudomonas aeruginosa PAO1, PA0148

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: add
Replicon chromosome
Genomic location 168213 - 169163 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_248838.1
GI 15595346
Affymetrix PA0148_at
Entrez 879249
GenBank AAG03538.1
INSDC AAG03538.1
NCBI Locus Tag PA0148
protein_id(GenBank) gb|AAG03538.1|AE004453_1|gnl|PseudoCAP|PA0148
UniParc UPI0000125510
UniProtKB Acc Q9I6Y4
UniRef100 UniRef100_B7V265
UniRef50 UniRef50_A6UXT7
UniRef90 UniRef90_A6UXT7


Feature Type CDS
Coding Frame 1
Product Name
adenine deaminase
Product Name Confidence: Class 3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.43
Kyte-Doolittle Hydrophobicity Value -0.275
Molecular Weight (kDa) 36.2
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3PAN HYDROLASE 10/19/10 The crystal structure of adenosine deaminase with hypoxanthine bound from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.627 X-RAY DIFFRACTION 99.7
3PBM HYDROLASE 10/20/10 The crystal structure of adenosine deaminase in complex with chloropurine from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.591 X-RAY DIFFRACTION 99.7
3OU8 HYDROLASE 09/14/10 The crystal structure of adenosine deaminase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.51 X-RAY DIFFRACTION 99.7
3PAO HYDROLASE 10/19/10 The crystal structure of adenosine deaminase with adenine bound from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.493 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 254 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000146 (532 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0148
Search term: adenine deaminase

Human Homologs

Ensembl 110, assembly GRCh38.p14
adenosine deaminase [Source:HGNC Symbol;Acc:HGNC:186]
E-value: 1.7e-21
Percent Identity: 23.6


Deduced amino acid sequence of Escherichia coli adenosine deaminase reveals evolutionarily conserved amino acid residues: implications for catalytic function.
Chang ZY, Nygaard P, Chinault AC, Kellems RE
Biochemistry 1991 Feb 26;30(8):2273-80
PubMed ID: 1998686
Pa0148 from Pseudomonas aeruginosa catalyzes the deamination of adenine.
Goble AM, Zhang Z, Sauder JM, Burley SK, Swaminathan S, Raushel FM
Biochemistry 2011 Aug 2;50(30):6589-97
PubMed ID: 21710971