Pseudomonas aeruginosa PAO1, PA0511 (nirJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Ontology

Ontology Accession Term GO Evidence Evidence Ontology (ECO) Code Reference Comments
Biological Process GO:0006783 heme biosynthetic process
IMP
Inferred from Mutant Phenotype
ECO:0000016
loss-of-function mutant phenotype evidence
8982003 Reviewed by curator
Biological Process GO:0006783 heme biosynthetic process
IDA
Inferred from Direct Assay
ECO:0000002
direct assay evidence
Biological Process GO:0051188 obsolete cofactor biosynthetic process
IDA
Inferred from Direct Assay
ECO:0000002
direct assay evidence
Biological Process GO:0006091 generation of precursor metabolites and energy
IDA
Inferred from Direct Assay
ECO:0000002
direct assay evidence
Molecular Function GO:0051536 iron-sulfur cluster binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF04055
ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0003824 catalytic activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF04055
ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0051539 4 iron, 4 sulfur cluster binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:TIGR04051
ECO:0000259
match to InterPro signature evidence used in automatic assertion

Functional Classifications Manually Assigned by PseudoCAP

Biosynthesis of cofactors, prosthetic groups and carriers Other PAO1 genes in this class
Energy metabolism Other PAO1 genes in this class

Pathways

Database Xref Pathway Version Evidence PMID
PseudoCAP Biosynthesis of heme d1 ECO:0000037
not_recorded
PseudoCAP Denitrification ECO:0000037
not_recorded

Functional Predictions from Interpro

Analysis Accession Description Interpro Accession Interpro Description Amino Acid Start Amino Acid Stop E-value
CDD cd01335 Radical_SAM - - 31 214 2.24965E-25
PIRSF PIRSF037420 PqqE IPR017200 Coenzyme PQQ synthesis protein E-like 13 386 0.0
SFLD SFLDF00393 heme D1 biosynthesis (NirJ-like) IPR023992 Pre-heme d1 synthase NirJ 1 383 0.0
Pfam PF04055 Radical SAM superfamily IPR007197 Radical SAM 33 187 1.4E-25
SFLD SFLDG01216 thioether bond formation requiring one auxiliary iron-sulfur cluster - - 1 383 0.0
NCBIfam TIGR04051 JCVI: heme d1 biosynthesis radical SAM protein NirJ IPR023992 Pre-heme d1 synthase NirJ 26 378 0.0
SUPERFAMILY SSF102114 Radical SAM enzymes - - 23 351 1.96E-68
Pfam PF13353 4Fe-4S single cluster domain - - 28 138 1.7E-8
FunFam G3DSA:3.20.20.70:FF:000188 Mycofactocin radical SAM maturase MftC - - 22 331 1.6E-101
SMART SM00729 MiaB IPR006638 Elp3/MiaA/NifB-like, radical SAM core domain 27 230 5.8E-4
PANTHER PTHR11228 RADICAL SAM DOMAIN PROTEIN - - 25 370 8.8E-84
Gene3D G3DSA:3.20.20.70 Aldolase class I IPR013785 Aldolase-type TIM barrel 22 247 3.2E-47

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.