Pseudomonas aeruginosa PAO1, PA1120 (tpbB)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: yfiN
Replicon chromosome
Genomic location 1213195 - 1214502 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentOverexpression of PA1120 increases pellicle formation.


RefSeq NP_249811.1
GI 15596317
Affymetrix PA1120_at
Entrez 881684
GenBank AAG04509.1
INSDC AAG04509.1
NCBI Locus Tag PA1120
protein_id(GenBank) gb|AAG04509.1|AE004542_5|gnl|PseudoCAP|PA1120
UniParc UPI00000C525B
UniProtKB Acc Q9I4L5
UniRef100 UniRef100_W0YL25
UniRef50 UniRef50_G8Q662
UniRef90 UniRef90_W0YL25


Feature Type CDS
Coding Frame 1
Product Name
diguanylate cyclase TpbB
Product Name Confidence: Class 1
Synonyms YfiN
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.88
Kyte-Doolittle Hydrophobicity Value -0.082
Molecular Weight (kDa) 47.5
Isoelectric Point (pI) 6.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4IOB LYASE 01/07/13 Crystal structure of the GGDEF domain of PA1120 (YfiN or TpbB) from Pseudomonas aeruginosa at 2.7 Ang. Pseudomonas aeruginosa 2.78 X-RAY DIFFRACTION 100.0
7A7E LIGASE 08/28/20 Structure of a delta-N mutant - E232start - of PA1120 (TpbB or YfiN) from Pseudomonas aeruginosa (PAO1) comprising only the GGDEF domain Pseudomonas aeruginosa PAO1 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 457 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001080 (443 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1120
Search term: tpbB

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Detection of new genes in a bacterial genome using Markov models for three gene classes.
Borodovsky M, McIninch JD, Koonin EV, Rudd KE, Médigue C, Danchin A
Nucleic Acids Res 1995 Sep 11;23(17):3554-62
PubMed ID: 7567469
Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885).
Ueda A, Wood TK
PLoS Pathog 2009 Jun;5(6):e1000483
PubMed ID: 19543378
YfiBNR mediates cyclic di-GMP dependent small colony variant formation and persistence in Pseudomonas aeruginosa.
Malone JG, Jaeger T, Spangler C, Ritz D, Spang A, Arrieumerlou C, Kaever V, Landmann R, Jenal U
PLoS Pathog 2010 Mar 12;6(3):e1000804
PubMed ID: 20300602
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Kulasakara H, Lee V, Brencic A, Liberati N, Urbach J, Miyata S, Lee DG, Neely AN, Hyodo M, Hayakawa Y, Ausubel FM, Lory S
Proc Natl Acad Sci U S A 2006 Feb 21;103(8):2839-44
PubMed ID: 16477007