Pseudomonas aeruginosa PAO1, PA0004 (gyrB)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0004
|
| Name |
gyrB
|
| Replicon | chromosome |
| Genomic location | 4275 - 6695 (+ strand) |
Cross-References
| RefSeq | NP_064724.1 |
| GI | 15595202 |
| Affymetrix | PA0004_gyrB_at |
| Entrez | 879230 |
| GenBank | AAG03394.1 |
| INSDC | AAG03394.1 |
| NCBI Locus Tag | PA0004 |
| protein_id(GenBank) | gb|AAG03394.1|AE004440_4|gnl|PseudoCAP|PA0004 |
| TIGR | NTL03PA00005 |
| UniParc | UPI00000C4EE1 |
| UniProtKB Acc | Q9I7C2 |
| UniProtKB ID | GYRB_PSEAE |
| UniRef100 | UniRef100_Q9I7C2 |
| UniRef50 | UniRef50_P13364 |
| UniRef90 | UniRef90_Q9I7C2 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
DNA gyrase subunit B
|
| Synonyms | |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
| Charge (pH 7) | -15.76 |
| Kyte-Doolittle Hydrophobicity Value | -0.411 |
| Molecular Weight (kDa) | 90.2 |
| Isoelectric Point (pI) | 5.74 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 633 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000004 (553 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0004
Search term: gyrB
Search term: DNA gyrase subunit B
|
Human Homologs
|
Ensembl
107, assembly
GRCh38.p13
|
DNA topoisomerase II alpha [Source:HGNC Symbol;Acc:HGNC:11989]
E-value:
1.0e-30
Percent Identity:
23.8
|
References
|
PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains.
Yamamoto S, Harayama S
Appl. Environ. Microbiol. 1995 Mar;61(3):1104-9
PubMed ID: 7793912
|
|
Type II topoisomerase mutations in ciprofloxacin-resistant strains of Pseudomonas aeruginosa.
Mouneimné H, Robert J, Jarlier V, Cambau E
Antimicrob. Agents Chemother. 1999 Jan;43(1):62-6
PubMed ID: 9869566
|
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|