Pseudomonas aeruginosa PAO1, PA0076 (tagF1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0076
Name
tagF1
Replicon chromosome
Genomic location 90844 - 91524 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248766.1
GI 15595274
Affymetrix PA0076_at
DNASU PaCD00006034
Entrez 879335
GenBank AAG03466.1
INSDC AAG03466.1
NCBI Locus Tag PA0076
protein_id(GenBank) gb|AAG03466.1|AE004446_14|gnl|PseudoCAP|PA0076
TIGR NTL03PA00077
UniParc UPI00000C4F1D
UniProtKB Acc Q9I756
UniProtKB ID Q9I756_PSEAE
UniRef100 UniRef100_Q9I756
UniRef50 UniRef50_Q4K3P5
UniRef90 UniRef90_Q9I756

Product

Feature Type CDS
Coding Frame 1
Product Name
TagF1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.61
Kyte-Doolittle Hydrophobicity Value 0.146
Molecular Weight (kDa) 23.9
Isoelectric Point (pI) 4.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6IZ8 SIGNALING PROTEIN 12/18/18 Crystal Structure of TagF from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 99.6
2QNU STRUCTURAL GENOMICS, UNKNOWN FUNCTION 07/19/07 Crystal structure of PA0076 from Pseudomonas aeruginosa PAO1 at 2.05 A resolution Pseudomonas aeruginosa PAO1 2.05 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 49 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000075 (376 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0076
Search term: tagF1
Search term: TagF1

Human Homologs

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading) 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433