Pseudomonas aeruginosa PAO1, PA0156 (triA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: triA
Replicon chromosome
Genomic location 177307 - 178458 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_248846.1
GI 15595354
Affymetrix PA0156_at
Entrez 882149
GenBank AAG03546.1
INSDC AAG03546.1
NCBI Locus Tag PA0156
protein_id(GenBank) gb|AAG03546.1|AE004453_9|gnl|PseudoCAP|PA0156
UniParc UPI00000C4F6B
UniProtKB Acc Q9I6X6
UniRef100 UniRef100_U9HZG4
UniRef50 UniRef50_Q4KK51
UniRef90 UniRef90_W0YM83


Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriA
Product Name Confidence: Class 1
Synonyms probable RND efflux membrane fusion protein precursor
Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriA efflux membrane fusion protein precursor)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.61
Kyte-Doolittle Hydrophobicity Value -0.137
Molecular Weight (kDa) 40.4
Isoelectric Point (pI) 6.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
TriA TriA is a membrane protein that is fused to TriB and both are required for the triclosan efflux pump function of TriABC-OpmH in P. aeruginosa. [PMID:17720796] ARO:3003679 protein homolog model Perfect 716.5 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6VEJ TRANSPORT PROTEIN 01/02/20 TriABC transporter from Pseudomonas aeruginosa Pseudomonas aeruginosa 4.5 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 422 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000154 (998 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0156
Search term: triA

Human Homologs


Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins.
Mima T, Joshi S, Gomez-Escalada M, Schweizer HP
J Bacteriol 2007 Nov;189(21):7600-9
PubMed ID: 17720796