Pseudomonas aeruginosa PAO1, PA0183 (atsA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Reference) GCF_000006765.1|latest
Locus Tag	PA0183
Name	atsA
Replicon	chromosome
Genomic location	207923 - 209533 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_248873.1
GI	15595381
Affymetrix	PA0183_atsA_at
Entrez	879288
GenBank	AAG03573.1
INSDC	AAG03573.1
NCBI Locus Tag	PA0183
protein_id(GenBank)	gb|AAG03573.1|AE004456_4|gnl|PseudoCAP|PA0183
TIGR	NTL03PA00184
UniParc	UPI0000165EBE
UniProtKB Acc	P51691
UniProtKB ID	ARS_PSEAE
UniRef100	UniRef100_P51691
UniRef50	UniRef50_P51691
UniRef90	UniRef90_P51691

Product

Feature Type	CDS
Coding Frame	1
Product Name	arylsulfatase Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7)	-12.39
Kyte-Doolittle Hydrophobicity Value	-0.378
Molecular Weight (kDa)	59.9
Isoelectric Point (pI)	5.51

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4CXU	HYDROLASE	04/08/14	G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with 3-Br-Phenolphenylphosphonate	PSEUDOMONAS AERUGINOSA	2.03	X-RAY DIFFRACTION	98.7
1HDH	HYDROLASE	11/16/00	Arylsulfatase from Pseudomonas aeruginosa	Pseudomonas aeruginosa	1.3	X-RAY DIFFRACTION	99.8
5AJ9	HYDROLASE	02/20/15	G7 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa	PSEUDOMONAS AERUGINOSA	2	X-RAY DIFFRACTION	98.5
4CYS	HYDROLASE	04/14/14	G6 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid	PSEUDOMONAS AERUGINOSA	1.88	X-RAY DIFFRACTION	98.7
4CXS	HYDROLASE	04/08/14	G4 mutant of PAS, arylsulfatase from Pseudomonas aeruginosa, in complex with Phenylphosphonic acid	PSEUDOMONAS AERUGINOSA	2.3	X-RAY DIFFRACTION	98.7
4CXK	HYDROLASE	04/07/14	G9 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa	PSEUDOMONAS AERUGINOSA	1.86	X-RAY DIFFRACTION	98.1
4CYR	HYDROLASE	04/14/14	G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa	PSEUDOMONAS AERUGINOSA	1.72	X-RAY DIFFRACTION	98.7

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
2-Aminopropanedioic Acid	DB02289	DrugBank	5.1.4	P51691	Arylsulfatase	100.0	536	0.0e+00

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 242 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000181 (374 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA0183 Search term: atsA Search term: arylsulfatase

Human Homologs

Ensembl 107, assembly GRCh38.p13	ENSP00000264914 arylsulfatase B [Source:HGNC Symbol;Acc:HGNC:714] E-value: 3.3e-30 Percent Identity: 29.9
Ensembl 107, assembly GRCh38.p13	ENSP00000379455 arylsulfatase B [Source:HGNC Symbol;Acc:HGNC:714] E-value: 3.3e-30 Percent Identity: 29.9
Ensembl 107, assembly GRCh38.p13	ENSP00000456339 arylsulfatase B [Source:HGNC Symbol;Acc:HGNC:714] E-value: 3.3e-30 Percent Identity: 29.9

References

Purification and characterization of the arylsulfatase synthesized by Pseudomonas aeruginosa PAO during growth in sulfate-free medium and cloning of the arylsulfatase gene (atsA). Beil S, Kehrli H, James P, Staudenmann W, Cook AM, Leisinger T, Kertesz MA Eur. J. Biochem. 1995 Apr;229(2):385-94 PubMed ID: 7744061