Pseudomonas aeruginosa PAO1, PA0402 (pyrB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0402
Name
pyrB
Replicon chromosome
Genomic location 444687 - 445691 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249093.1
GI 15595599
Affymetrix PA0402_pyrB_at
Entrez 878267
GenBank AAG03791.1
INSDC AAG03791.1
NCBI Locus Tag PA0402
protein_id(GenBank) gb|AAG03791.1|AE004477_8|gnl|PseudoCAP|PA0402
TIGR NTL03PA00403
UniParc UPI0000132C39
UniProtKB Acc Q59653
UniProtKB ID PYRB_PSEAE
UniRef100 UniRef100_B7V3Y9
UniRef50 UniRef50_Q59711
UniRef90 UniRef90_B7V3Y9

Product

Feature Type CDS
Coding Frame 1
Product Name
aspartate carbamoyltransferase
Product Name Confidence: Class 2
Synonyms aspartate transcarbamoylase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.89
Kyte-Doolittle Hydrophobicity Value -0.114
Molecular Weight (kDa) 36.6
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 646 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000393 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0402
Search term: pyrB

Human Homologs

References

Aspartate transcarbamoylase genes of Pseudomonas putida: requirement for an inactive dihydroorotase for assembly into the dodecameric holoenzyme.
Schurr MJ, Vickrey JF, Kumar AP, Campbell AL, Cunin R, Benjamin RC, Shanley MS, O'Donovan GA
J Bacteriol 1995 Apr;177(7):1751-9
PubMed ID: 7896697
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994