Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0595
|
Name |
lptD
Synonym: imp Synonym: ostA |
Replicon | chromosome |
Genomic location | 653753 - 656527 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
Comment |
RefSeq | NP_249286.1 |
GI | 15595792 |
Affymetrix | PA0595_ostA_at |
Entrez | 880756 |
GenBank | AAG03984.1 |
INSDC | AAG03984.1 |
NCBI Locus Tag | PA0595 |
protein_id(GenBank) | gb|AAG03984.1|AE004495_8|gnl|PseudoCAP|PA0595 |
TIGR | NTL03PA00596 |
UniParc | UPI0000130ED1 |
UniProtKB Acc | Q9I5U2 |
UniProtKB ID | LPTD_PSEAE |
UniRef100 | UniRef100_Q9I5U2 |
UniRef50 | UniRef50_Q88A43 |
UniRef90 | UniRef90_Q9I5U2 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
LPS-assembly protein LptD
|
Synonyms |
organic solvent tolerance protein OstA precursor |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (104.27 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -13.50 |
Kyte-Doolittle Hydrophobicity Value | -0.599 |
Molecular Weight (kDa) | 104.3 |
Isoelectric Point (pI) | 5.29 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5IVA | TRANSPORT PROTEIN | 03/20/16 | The LPS Transporter LptDE from Pseudomonas aeruginosa, core complex | Pseudomonas aeruginosa | 2.988 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 254 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000578 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0595
Search term: lptD
Search term: LPS-assembly protein LptD
|
Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli.
Aono R, Negishi T, Nakajima H
Appl Environ Microbiol 1994 Dec;60(12):4624-6
PubMed ID: 7811102
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Peptidomimetic antibiotics target outer-membrane biogenesis in Pseudomonas aeruginosa.
Srinivas N, Jetter P, Ueberbacher BJ, Werneburg M, Zerbe K, Steinmann J, Van der Meijden B, Bernardini F, Lederer A, Dias RL, Misson PE, Henze H, Zumbrunn J, Gombert FO, Obrecht D, Hunziker P, Schauer S, Ziegler U, Käch A, Eberl L, Riedel K, DeMarco SJ, Robinson JA
Science 2010 Feb 19;327(5968):1010-3
PubMed ID: 20167788
|
Outer-membrane protein LptD (PA0595) plays a role in the regulation of alginate synthesis in Pseudomonas aeruginosa.
Pandey S, Delgado C, Kumari H, Florez L, Mathee K
J Med Microbiol 2018 Aug;67(8):1139-1156
PubMed ID: 29923820
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|