Pseudomonas aeruginosa PAO1, PA0956 (proS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0956
Name
proS
Replicon chromosome
Genomic location 1041689 - 1043404 (- strand)

Cross-References

RefSeq NP_249647.1
GI 15596153
Affymetrix PA0956_proS_at
Entrez 879563
GenBank AAG04345.1
INSDC AAG04345.1
NCBI Locus Tag PA0956
protein_id(GenBank) gb|AAG04345.1|AE004529_11|gnl|PseudoCAP|PA0956
TIGR NTL03PA00957
UniParc UPI00000C51DC
UniProtKB Acc Q9I502
UniProtKB ID SYP_PSEAE
UniRef100 UniRef100_B7UXX6
UniRef50 UniRef50_P43830
UniRef90 UniRef90_B7UXX6

Product

Feature Type CDS
Coding Frame 1
Product Name
prolyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms proline aminoacyl tRNA synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -15.16
Kyte-Doolittle Hydrophobicity Value -0.194
Molecular Weight (kDa) 63.1
Isoelectric Point (pI) 5.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UCM LIGASE 12/22/16 Crystal Structure of Prolyl-tRNA Synthetase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 665 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000922 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0956
Search term: proS

Human Homologs

Ensembl 110, assembly GRCh38.p14
prolyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:30563]
E-value: 9.1e-66
Percent Identity: 32.3

References

Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs.
Eriani G, Delarue M, Poch O, Gangloff J, Moras D
Nature 1990 Sep 13;347(6289):203-6
PubMed ID: 2203971
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994