Pseudomonas aeruginosa UCBPP-PA14, PA14_03050 (pobA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_03050
Name	pobA
Replicon	chromosome
Genomic location	269376 - 270560 (- strand)
Transposon Mutants	1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	YP_788398.1
GI	116053960
EC Number	1.14.13.2
Entrez	4383658
INSDC	ABJ15196.1
NCBI Locus Tag	PA14_03050
UniParc	UPI000045D3B9
UniProtKB Acc	Q02UI8
UniProtKB ID	Q02UI8_PSEAB
UniRef100	UniRef100_Q02UI8
UniRef50	UniRef50_P20586
UniRef90	UniRef90_P20586

Product

Feature Type	CDS
Coding Frame	1
Product Name	4-hydroxybenzoate 3-monooxygenase
Synonyms
Evidence for Translation
Charge (pH 7)	0.03
Kyte-Doolittle Hydrophobicity Value	-0.252
Molecular Weight (kDa)	44.3
Isoelectric Point (pI)	7.01

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
1IUV	OXIDOREDUCTASE	11/22/95	P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-4-HYDROXYBENZOATE AT PH 5.0	Pseudomonas aeruginosa	2.5	X-RAY DIFFRACTION	99.2
1K0L	HYDROLASE	09/19/01	Pseudomonas aeruginosa phbh R220Q free of p-OHB	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	99.0
1K0J	HYDROLASE	09/19/01	Pseudomonas aeruginosa phbh R220Q in complex with NADPH and free of p-OHB	Pseudomonas aeruginosa	2.2	X-RAY DIFFRACTION	99.0
1PBB	OXIDOREDUCTASE	07/06/94	CRYSTAL STRUCTURES OF WILD-TYPE P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE, 2,4-DIHYDROXYBENZOATE AND 2-HYDROXY-4-AMINOBENZOATE AND OF THE TRY222ALA MUTANT, COMPLEXED WITH 2-HYDROXY-4-AMINOBENZOATE. EVIDENCE FOR A PROTON CHANNEL AND A NEW BINDING MODE OF THE FLAVIN RING	Pseudomonas fluorescens	2.5	X-RAY DIFFRACTION	99.5
1D7L	OXIDOREDUCTASE	10/18/99	STRUCTURE-FUNCTION CORRELATIONS OF THE REACTION OF REDUCED NICOTINAMIDE ANALOGS WITH P-HYDROXYBENZOATE HYDROXYLASE SUBSTITUTED WITH A SERIES OF 8-SUBSTITUTED FLAVINS	Pseudomonas aeruginosa	2.2	X-RAY DIFFRACTION	99.2
1IUT	OXIDOREDUCTASE	11/22/95	P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE AT PH 7.4	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	99.2
1PDH	OXIDOREDUCTASE	12/01/94	CRYSTAL STRUCTURE OF P-HYDROXYBENZOATE HYDROXYLASE RECONSTITUTED WITH THE MODIFIED FAD PRESENT IN ALCOHOL OXIDASE FROM METHYLOTROPHIC YEASTS: EVIDENCE FOR AN ARABINOFLAVIN	Pseudomonas fluorescens	2.1	X-RAY DIFFRACTION	99.7
1K0I	HYDROLASE	09/19/01	Pseudomonas aeruginosa phbh R220Q in complex with 100mM PHB	Pseudomonas aeruginosa	1.8	X-RAY DIFFRACTION	99.0
1IUS	OXIDOREDUCTASE	11/22/95	P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE AT PH 5.0	Pseudomonas aeruginosa	2.2	X-RAY DIFFRACTION	99.2
1YKJ	OXIDOREDUCTASE	01/18/05	A45G p-hydroxybenzoate hydroxylase with p-hydroxybenzoate bound	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	99.0
1PBC	OXIDOREDUCTASE	07/06/94	CRYSTAL STRUCTURES OF WILD-TYPE P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE, 2,4-DIHYDROXYBENZOATE AND 2-HYDROXY-4-AMINOBENZOATE AND OF THE TRY222ALA MUTANT, COMPLEXED WITH 2-HYDROXY-4-AMINOBENZOATE. EVIDENCE FOR A PROTON CHANNEL AND A NEW BINDING MODE OF THE FLAVIN RING	Pseudomonas fluorescens	2.8	X-RAY DIFFRACTION	99.5
1IUX	OXIDOREDUCTASE	11/22/95	P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-4-HYDROXYBENZOATE AT PH 9.4	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	99.2
1DOD	OXIDOREDUCTASE	09/06/94	THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS	Pseudomonas aeruginosa	2.1	X-RAY DIFFRACTION	99.2
1DOE	OXIDOREDUCTASE	09/06/94	THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS	Pseudomonas aeruginosa	2.3	X-RAY DIFFRACTION	99.2
1DOC	OXIDOREDUCTASE	09/06/94	THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	99.2
2PHH	OXIDOREDUCTASE	06/19/89	THE COENZYME ANALOGUE ADENOSINE 5-DIPHOSPHORIBOSE DISPLACES FAD IN THE ACTIVE SITE OF P-HYDROXYBENZOATE HYDROXYLASE. AN X-RAY CRYSTALLOGRAPHIC INVESTIGATION	Pseudomonas fluorescens	2.7	X-RAY DIFFRACTION	99.7
1PBE	OXIDOREDUCTASE	07/06/94	CRYSTAL STRUCTURE OF THE P-HYDROXYBENZOATE HYDROXYLASE-SUBSTRATE COMPLEX REFINED AT 1.9 ANGSTROMS RESOLUTION. ANALYSIS OF THE ENZYME-SUBSTRATE AND ENZYME-PRODUCT COMPLEXES	Pseudomonas fluorescens	1.9	X-RAY DIFFRACTION	99.7
1PBD	OXIDOREDUCTASE	07/06/94	CRYSTAL STRUCTURES OF WILD-TYPE P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE, 2,4-DIHYDROXYBENZOATE AND 2-HYDROXY-4-AMINOBENZOATE AND OF THE TRY222ALA MUTANT, COMPLEXED WITH 2-HYDROXY-4-AMINOBENZOATE. EVIDENCE FOR A PROTON CHANNEL AND A NEW BINDING MODE OF THE FLAVIN RING	Pseudomonas fluorescens	2.3	X-RAY DIFFRACTION	99.5
1PXB	OXIDOREDUCTASE	09/27/94	CRYSTAL STRUCTURES OF MUTANT PSEUDOMONAS AERUGINOSA P-HYDROXYBENZOATE HYDROXYLASE: THE TYR201PHE, TYR385PHE, AND ASN300ASP VARIANTS	Pseudomonas aeruginosa	2.3	X-RAY DIFFRACTION	99.0
6JU1	OXIDOREDUCTASE	04/12/19	p-Hydroxybenzoate hydroxylase Y385F mutant complexed with 3,4-dihydroxybenzoate	Pseudomonas aeruginosa	1.6	X-RAY DIFFRACTION	99.0
1DOB	OXIDOREDUCTASE	09/06/94	THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	99.0
1IUU	OXIDOREDUCTASE	11/22/95	P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE AT PH 9.4	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	99.2
1IUW	OXIDOREDUCTASE	11/22/95	P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-4-HYDROXYBENZOATE AT PH 7.4	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	99.2
1PHH	OXIDOREDUCTASE	11/04/87	CRYSTAL STRUCTURE OF P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH ITS REACTION PRODUCT 3,4-DIHYDROXYBENZOATE	Pseudomonas fluorescens	2.3	X-RAY DIFFRACTION	99.7

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
P-Hydroxybenzoic Acid	DB04242	DrugBank	5.1.4	P00438	p-hydroxybenzoate hydroxylase	99.7	394	1.7e-228
2,4-Dihydroxybenzoic Acid	DB02839	DrugBank	5.1.4	P00438	p-hydroxybenzoate hydroxylase	99.7	394	1.7e-228
Aminobenzoic acid	DB02362	DrugBank	5.1.4	P00438	p-hydroxybenzoate hydroxylase	99.7	394	1.7e-228
Adenosine-5-Diphosphoribose	DB02059	DrugBank	5.1.4	P00438	p-hydroxybenzoate hydroxylase	99.7	394	1.7e-228
Flavin adenine dinucleotide	DB03147	DrugBank	5.1.4	P00438	p-hydroxybenzoate hydroxylase	99.7	394	1.7e-228

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 152 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000245 (499 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_03050 Search term: pobA Search term: 4-hydroxybenzoate 3-monooxygenase

Human Homologs

References