Pseudomonas aeruginosa PAO1, PA3997 (lipB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3997
Name
lipB
Replicon chromosome
Genomic location 4477974 - 4478627 (- strand)

Cross-References

RefSeq NP_252686.1
GI 15599192
Affymetrix PA3997_lipB_at
DNASU PaCD00007746
Entrez 878952
GenBank AAG07384.1
INSDC AAG07384.1
NCBI Locus Tag PA3997
protein_id(GenBank) gb|AAG07384.1|AE004817_8|gnl|PseudoCAP|PA3997
TIGR NTL03PA03997
UniParc UPI000012E6F9
UniProtKB Acc Q9X6V9
UniProtKB ID LIPB_PSEAE
UniRef100 UniRef100_A6V0B3
UniRef50 UniRef50_Q88DM4
UniRef90 UniRef90_A6V0B3

Product

Feature Type CDS
Coding Frame 1
Product Name
lipoate-protein ligase B
Product Name Confidence: Class 2
Synonyms lipoate biosynthesis protein B
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.90
Kyte-Doolittle Hydrophobicity Value 0.001
Molecular Weight (kDa) 23.9
Isoelectric Point (pI) 6.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 430 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001686 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3997
Search term: lipB

Human Homologs

References

Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes.
Reed KE, Cronan JE Jr
J Bacteriol 1993 Mar;175(5):1325-36
PubMed ID: 8444795