Pseudomonas aeruginosa PAO1, PA4000 (rlpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4000
Name
rlpA
Replicon chromosome
Genomic location 4480205 - 4481233 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Commentseptal ring protein, suppressor of prc, minor lipoprotein

Cross-References

RefSeq NP_252689.1
GI 15599195
Affymetrix PA4000_at
Entrez 878957
GenBank AAG07387.1
INSDC AAG07387.1
NCBI Locus Tag PA4000
protein_id(GenBank) gb|AAG07387.1|AE004817_11|gnl|PseudoCAP|PA4000
TIGR NTL03PA04000
UniParc UPI000013427D
UniProtKB Acc Q9X6V6
UniProtKB ID RLPA_PSEAE
UniRef100 UniRef100_Q9X6V6
UniRef50 UniRef50_Q9X6V6
UniRef90 UniRef90_Q9X6V6

Product

Feature Type CDS
Coding Frame 1
Product Name
RlpA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 13.37
Kyte-Doolittle Hydrophobicity Value -0.193
Molecular Weight (kDa) 36.5
Isoelectric Point (pI) 10.10

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6I09 CELL CYCLE 10/25/18 Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa in complex with denuded glycan obtained by soaking Pseudomonas aeruginosa 1.48 X-RAY DIFFRACTION 100.0
6I0A CELL CYCLE 10/25/18 Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa in complex with nuded glycan obtained by co-crystallization Pseudomonas aeruginosa 1.3 X-RAY DIFFRACTION 100.0
6I0N CELL CYCLE 10/26/18 Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa in complex with denuded glycan ended in anhNAM Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0
6I05 CELL CYCLE 10/25/18 Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.213 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 349 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001683 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4000
Search term: rlpA
Search term: RlpA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon.
Matsuzawa H, Asoh S, Kunai K, Muraiso K, Takasuga A, Ohta T
J Bacteriol 1989 Jan;171(1):558-60
PubMed ID: 2644207
Discovery and characterization of three new Escherichia coli septal ring proteins that contain a SPOR domain: DamX, DedD, and RlpA.
Arends SJ, Williams K, Scott RJ, Rolong S, Popham DL, Weiss DS
J Bacteriol 2010 Jan;192(1):242-55
PubMed ID: 19880599