Pseudomonas aeruginosa PAO1, PA4209 (phzM)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA4209
|
| Name |
phzM
|
| Replicon | chromosome |
| Genomic location | 4712095 - 4713099 (- strand) |
| Transposon Mutants | 3 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_252898.1 |
| GI | 15599404 |
| Affymetrix | PA4209_at |
| DNASU | PaCD00007828 |
| Entrez | 880514 |
| GenBank | AAG07596.1 |
| INSDC | AAG07596.1 |
| NCBI Locus Tag | PA4209 |
| protein_id(GenBank) | gb|AAG07596.1|AE004837_7|gnl|PseudoCAP|PA4209 |
| TIGR | NTL03PA04209 |
| UniParc | UPI00000C5C75 |
| UniProtKB Acc | Q9HWH2 |
| UniProtKB ID | Q9HWH2_PSEAE |
| UniRef100 | UniRef100_W0YZX9 |
| UniRef50 | UniRef50_W0YZX9 |
| UniRef90 | UniRef90_W0YZX9 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
probable phenazine-specific methyltransferase
|
| Synonyms |
probable O-methyltransferase |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -11.69 |
| Kyte-Doolittle Hydrophobicity Value | 0.041 |
| Molecular Weight (kDa) | 36.4 |
| Isoelectric Point (pI) | 4.77 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 2IP2 | TRANSFERASE | 10/11/06 | Structure of the Pyocyanin Biosynthetic Protein PhzM | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
Virulence Evidence
| Source 1 | |
| Database | Victors : 4248 |
| Category | Phenazine biosynthesis |
| Evidence |
ECO:0000015
mutant phenotype evidence
|
| Host Organism | Mus musculus |
| Notes | |
| Reference | 15213173 |
| Source 2 | |
| Database | VFDB : VF0100 |
| Category | Phenazine biosynthesis |
| Evidence |
|
| Host Organism | |
| Notes | |
| Reference | |
| Source 3 | |
| Database | PseudoCAP |
| Category | Phenazine biosynthesis |
| Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
| Host Organism | Mus musculus |
| Notes | |
| Infection model | chronic pulmonary Infection model |
| Infection model | acute pulmonary Infection model |
| Reference | 15213173 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 122 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG001498 (298 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA4209
Search term: phzM
Search term: probable phenazine-specific methyltransferase
|
Human Homologs
References
|
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
J Bacteriol 2001 Nov;183(21):6454-65
PubMed ID: 11591691
|