Pseudomonas aeruginosa PAO1, PA4240 (rpsK)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA4240
|
| Name |
rpsK
|
| Replicon | chromosome |
| Genomic location | 4756084 - 4756473 (- strand) |
Cross-References
| RefSeq | NP_252930.1 |
| GI | 15599436 |
| Affymetrix | PA4240_rpsK_at |
| Entrez | 881817 |
| GenBank | AAG07628.1 |
| INSDC | AAG07628.1 |
| NCBI Locus Tag | PA4240 |
| protein_id(GenBank) | gb|AAG07628.1|AE004841_6|gnl|PseudoCAP|PA4240 |
| TIGR | NTL03PA04241 |
| UniParc | UPI0000134C06 |
| UniProtKB Acc | Q9HWF8 |
| UniProtKB ID | RS11_PSEAE |
| UniRef100 | UniRef100_C1DKN6 |
| UniRef50 | UniRef50_P10789 |
| UniRef90 | UniRef90_C1DKN6 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
30S ribosomal protein S11
|
| Synonyms | |
| Evidence for Translation |
Detected using LC-MS/MS (13.63 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | 15.64 |
| Kyte-Doolittle Hydrophobicity Value | -0.437 |
| Molecular Weight (kDa) | 13.6 |
| Isoelectric Point (pI) | 11.48 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 7UNV | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.7 | ELECTRON MICROSCOPY | 100.0 |
| 6SPC | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.95 | ELECTRON MICROSCOPY | 100.0 |
| 7UNU | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
| 6SPF | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.89 | ELECTRON MICROSCOPY | 100.0 |
| 6SPE | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 30s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.6 | ELECTRON MICROSCOPY | 100.0 |
| 7UNR | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
| 6SPG | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.34 | ELECTRON MICROSCOPY | 100.0 |
| 7UNW | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 100.0 |
Drugs Targeting this Protein
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
| Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
| Plazomicin | DB12615 | DrugBank | 5.1.4 | L0LZJ6 | 30S ribosomal protein S11 | 79.8 | 129 | 9.2e-54 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 664 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG001470 (536 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA4240
Search term: rpsK
Search term: 30S ribosomal protein S11
|
Human Homologs
|
Ensembl
110, assembly
GRCh38.p14
|
mitochondrial ribosomal protein S11 [Source:HGNC Symbol;Acc:HGNC:14050]
E-value:
5.0e-20
Percent Identity:
47.3
|
References
|
Primary structure of protein S11 from Escherichia coli ribosomes.
Kamp R, Wittmann-Liebold B
FEBS Lett 1980 Nov 17;121(1):117-22
PubMed ID: 7007074
|
|
Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli.
Bedwell D, Davis G, Gosink M, Post L, Nomura M, Kestler H, Zengel JM, Lindahl L
Nucleic Acids Res 1985 Jun 11;13(11):3891-903
PubMed ID: 2989779
|
|
Photoaffinity labeling of 30S-subunit proteins S7 and S11 by 4-thiouridine-substituted tRNA(Phe) situated at the P site of Escherichia coli ribosomes.
Rosen KV, Zimmerman RA
RNA 1997 Sep;3(9):1028-36
PubMed ID: 9292501
|
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|