Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4249
|
Name |
rpsH
|
Replicon | chromosome |
Genomic location | 4760480 - 4760872 (- strand) |
RefSeq | NP_252939.1 |
GI | 15599445 |
Affymetrix | PA4249_rpsH_at |
DNASU | PaCD00007844 |
Entrez | 881738 |
GenBank | AAG07637.1 |
INSDC | AAG07637.1 |
NCBI Locus Tag | PA4249 |
protein_id(GenBank) | gb|AAG07637.1|AE004841_15|gnl|PseudoCAP|PA4249 |
TIGR | NTL03PA04250 |
UniParc | UPI0000135176 |
UniProtKB Acc | Q9HWE9 |
UniProtKB ID | RS8_PSEAE |
UniRef100 | UniRef100_A6UZK2 |
UniRef50 | UniRef50_A6UZK2 |
UniRef90 | UniRef90_A6UZK2 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
30S ribosomal protein S8
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 5.96 |
Kyte-Doolittle Hydrophobicity Value | -0.115 |
Molecular Weight (kDa) | 14.2 |
Isoelectric Point (pI) | 10.19 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7UNR | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
7UNU | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
6SPF | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.89 | ELECTRON MICROSCOPY | 99.2 |
7UNV | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.7 | ELECTRON MICROSCOPY | 100.0 |
6SPG | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.34 | ELECTRON MICROSCOPY | 100.0 |
7UNW | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 100.0 |
6SPE | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 30s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.6 | ELECTRON MICROSCOPY | 100.0 |
6SPC | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.95 | ELECTRON MICROSCOPY | 98.4 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 665 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001461 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4249
Search term: rpsH
Search term: 30S ribosomal protein S8
|
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M
Nucleic Acids Res 1983 May 11;11(9):2599-616
PubMed ID: 6222285
|
Mutagenesis of ribosomal protein S8 from Escherichia coli: defects in regulation of the spc operon.
Wower I, Kowaleski MP, Sears LE, Zimmermann RA
J Bacteriol 1992 Feb;174(4):1213-21
PubMed ID: 1735715
|
Structural features of the binding site for ribosomal protein S8 in Escherichia coli 16S rRNA defined using NMR spectroscopy.
Kalurachchi K, Uma K, Zimmermann RA, Nikonowicz EP
Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2139-44
PubMed ID: 9122161
|