Pseudomonas aeruginosa PAO1, PA4250 (rpsN)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4250
Name	rpsN
Replicon	chromosome
Genomic location	4761062 - 4761367 (- strand)

Cross-References

RefSeq	NP_252940.1
GI	15599446
Affymetrix	PA4250_rpsN_at
DNASU	PaCD00007845
Entrez	881778
GenBank	AAG07638.1
INSDC	AAG07638.1
NCBI Locus Tag	PA4250
protein_id(GenBank)	gb|AAG07638.1|AE004841_16|gnl|PseudoCAP|PA4250
TIGR	NTL03PA04251
UniParc	UPI0000134CE7
UniProtKB Acc	Q9HWE8
UniProtKB ID	RS14_PSEAE
UniRef100	UniRef100_A6UZK1
UniRef50	UniRef50_A6UZK1
UniRef90	UniRef90_A6UZK1

Product

Feature Type	CDS
Coding Frame	1
Product Name	30S ribosomal protein S14 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	17.19
Kyte-Doolittle Hydrophobicity Value	-0.920
Molecular Weight (kDa)	11.6
Isoelectric Point (pI)	11.90

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6SPF	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.89	ELECTRON MICROSCOPY	100.0
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0
6SPG	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.34	ELECTRON MICROSCOPY	100.0
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPC	RIBOSOME	09/01/19	Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.95	ELECTRON MICROSCOPY	100.0
6SPE	RIBOSOME	09/01/19	Pseudomonas aeruginosa 30s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.6	ELECTRON MICROSCOPY	100.0
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 583 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001460 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4250 Search term: rpsN Search term: 30S ribosomal protein S14

Human Homologs

References

The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M Nucleic Acids Res 1983 May 11;11(9):2599-616 PubMed ID: 6222285

Reconstitution of Escherichia coli ribosomes containing puromycin-modified S14: functional effects of the photoaffinity labeling of a protein essential for tRNA binding. Kerlavage AR, Cooperman BS Biochemistry 1986 Dec 2;25(24):8002-10 PubMed ID: 3542041